FastQCFastQC Report
Thu 29 Jun 2023
SRR3312949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3312949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32132108
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG8670192.698294802195984Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT8432422.624297167182433RNA PCR Primer, Index 1 (100% over 25bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG3950051.2293155494186687Illumina Small RNA Adapter 2 (100% over 21bp)
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC2176980.6775092378003957RNA PCR Primer, Index 1 (100% over 27bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA2088210.6498826656501964RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC2077020.64640016770764RNA PCR Primer, Index 1 (100% over 24bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGGGTGCCAAGGA1949320.6066579883274387RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGTGCCAAGGAAC1747820.5439481281464633RNA PCR Primer, Index 1 (100% over 24bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC1604180.4992451786854445RNA PCR Primer, Index 1 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT1585470.49342234253663037RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC1376530.4283970413643574RNA PCR Primer, Index 1 (100% over 26bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGA1330450.4140562455472887RNA PCR Primer, Index 1 (100% over 22bp)
GAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTC1123830.34975296360886127RNA PCR Primer, Index 1 (100% over 26bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC830640.25850778293163956RNA PCR Primer, Index 1 (100% over 24bp)
CCCACTAATAGGGAACGTGAGCTTGGAATTCTCGGGTGCCAAGGAACTCC793660.24699904531629235RNA PCR Primer, Index 1 (100% over 27bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA758030.2359104482033983RNA PCR Primer, Index 1 (100% over 23bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC732690.22802425536475854RNA PCR Primer, Index 1 (100% over 24bp)
ATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACT726520.22610405766095393RNA PCR Primer, Index 1 (100% over 25bp)
ATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAACT688670.21432456283291468RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAATGGAATTCTCGGGTGCCAAGGAA679640.21151428969428338RNA PCR Primer, Index 1 (100% over 23bp)
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTGCCAAGGAACT673980.20975281173585RNA PCR Primer, Index 1 (100% over 25bp)
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGTGCCAAGGAAC647810.20160831029199824RNA PCR Primer, Index 1 (100% over 24bp)
AAGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAA642990.2001082530906469RNA PCR Primer, Index 1 (100% over 23bp)
AGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAACT629390.1958757265474148RNA PCR Primer, Index 1 (100% over 25bp)
AAAATATGTACAGTAAAATTGTAAGTGGAATTCTCGGGTGCCAAGGAACT551450.17161961487245095RNA PCR Primer, Index 1 (100% over 25bp)
TCGAAGAGTCGAGTTGTTTGGGAATGGAATTCTCGGGTGCCAAGGAACTC551140.17152313816448023RNA PCR Primer, Index 1 (100% over 26bp)
AGGGTGAGAATCCCGTGTGGCGAGGATGGAATTCTCGGGTGCCAAGGAAC541490.16851991160990745RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGCGTCGCTGAACCATAGCAGTGGAATTCTCGGGTGCCAAGGAACTC534410.16631650808593074RNA PCR Primer, Index 1 (100% over 26bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA519030.16153001850983448RNA PCR Primer, Index 1 (100% over 23bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGAA509650.15861082005575233RNA PCR Primer, Index 1 (100% over 23bp)
AATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAAC499380.1554146400852381RNA PCR Primer, Index 1 (100% over 24bp)
ACCTGCGGAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC494730.15396748946567715RNA PCR Primer, Index 1 (100% over 31bp)
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCTCGGGTGCCAA457060.14224401337129827No Hit
GAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAACT435790.13562446634375808RNA PCR Primer, Index 1 (100% over 25bp)
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGGTGCCAAGGAA435690.13559334482505786RNA PCR Primer, Index 1 (100% over 23bp)
TCGAAGAGTCGAGTTGTTTGGGAATTGGAATTCTCGGGTGCCAAGGAACT431240.13420843724289735RNA PCR Primer, Index 1 (100% over 25bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT428710.1334210628197814RNA PCR Primer, Index 1 (100% over 25bp)
GAGGGTGAGAATCCCGTGTGGCGAGGATGGAATTCTCGGGTGCCAAGGAA426570.13275506231959633RNA PCR Primer, Index 1 (100% over 23bp)
GATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAAC422420.13146351929353653RNA PCR Primer, Index 1 (100% over 24bp)
TGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAA386370.1202442118021015RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCTGAGATCTCGTATGC379140.11799412600007443RNA PCR Primer, Index 25 (97% over 38bp)
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGGTGCCAAGGAA363630.11316717844966784RNA PCR Primer, Index 1 (100% over 23bp)
TATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTC354610.11036001746290657RNA PCR Primer, Index 1 (100% over 26bp)
TTTGATGATTCATAATAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC347290.10808192229404931RNA PCR Primer, Index 1 (100% over 31bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCCAAG345580.10754974432427528No Hit
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAAGGA330410.10282860993744948RNA PCR Primer, Index 1 (100% over 22bp)
GAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAA326500.10161175855627026RNA PCR Primer, Index 1 (100% over 23bp)
CGATCAACTTAGAACTGGTACGGACAATGGAATTCTCGGGTGCCAAGGAA322980.10051628109802196RNA PCR Primer, Index 1 (100% over 23bp)
AGGGTGAGAATCCCGTGTGGCGAGGAGTGGAATTCTCGGGTGCCAAGGAA321980.10020506591101959RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)