FastQCFastQC Report
Thu 29 Jun 2023
SRR3312950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3312950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30475243
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT12741894.18106264156778RNA PCR Primer, Index 1 (100% over 25bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG7833352.5703978800103418Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC5167741.69571740576441RNA PCR Primer, Index 1 (100% over 24bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGGGTGCCAAGGA2811090.9224175833478999RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT2422800.7950059659901646RNA PCR Primer, Index 1 (100% over 25bp)
CCCACTAATAGGGAACGTGAGCTTGGAATTCTCGGGTGCCAAGGAACTCC2275470.7466618067655769RNA PCR Primer, Index 1 (100% over 27bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGA1708590.5606485237869966RNA PCR Primer, Index 1 (100% over 22bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG1705140.5595164573421121Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGTGCCAAGGAAC1577410.5176037480652739RNA PCR Primer, Index 1 (100% over 24bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA1548320.5080582950560886RNA PCR Primer, Index 1 (100% over 22bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC1379620.4527018865772457RNA PCR Primer, Index 1 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC1321850.4337455159914558RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCGGGATCTCGTATGC1256370.4122592230027501RNA PCR Primer, Index 46 (97% over 38bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC1103410.3620676625941916RNA PCR Primer, Index 1 (100% over 24bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA1081160.3547666543626904RNA PCR Primer, Index 1 (100% over 23bp)
ATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACT1031680.33853052459663735RNA PCR Primer, Index 1 (100% over 25bp)
AGGGTGAGAATCCCGTGTGGCGAGGATGGAATTCTCGGGTGCCAAGGAAC1017820.3339825707050146RNA PCR Primer, Index 1 (100% over 24bp)
GAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTC1010530.3315904650866935RNA PCR Primer, Index 1 (100% over 26bp)
ATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAACT980600.32176937850831905RNA PCR Primer, Index 1 (100% over 25bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC826230.27111514746576426RNA PCR Primer, Index 1 (100% over 24bp)
AGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAACT818520.26858522506284854RNA PCR Primer, Index 1 (100% over 25bp)
GAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAACT784460.2574089401026269RNA PCR Primer, Index 1 (100% over 25bp)
GAGGGTGAGAATCCCGTGTGGCGAGGATGGAATTCTCGGGTGCCAAGGAA762760.25028840623190435RNA PCR Primer, Index 1 (100% over 23bp)
AGGGTGAGAATCCCGTGTGGCGAGGAGTGGAATTCTCGGGTGCCAAGGAA760790.2496419798851153RNA PCR Primer, Index 1 (100% over 23bp)
AATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAAC703050.2306954533553678RNA PCR Primer, Index 1 (100% over 24bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT680260.2232172521151021RNA PCR Primer, Index 1 (100% over 25bp)
AAGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAA653940.21458073361383861RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGCGTCGCTGAACCATAGCAGTGGAATTCTCGGGTGCCAAGGAACTC593250.19466620823991462RNA PCR Primer, Index 1 (100% over 26bp)
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGGTGCCAAGGAA589700.19350132827488856RNA PCR Primer, Index 1 (100% over 23bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGAA586550.19246770239042885RNA PCR Primer, Index 1 (100% over 23bp)
GATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAAC544030.17851539362622967RNA PCR Primer, Index 1 (100% over 24bp)
TCGAAGAGTCGAGTTGTTTGGGAATTGGAATTCTCGGGTGCCAAGGAACT534790.1754834243651478RNA PCR Primer, Index 1 (100% over 25bp)
GCCAGCTGGGACTGAGGACTGCGACGTGGAATTCTCGGGTGCCAAGGAAC511170.16773287090770694RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGGCAGGATAAATCCATAGGTGGAATTCTCGGGTGCCAAGGAACT508350.16680752963971446RNA PCR Primer, Index 1 (100% over 25bp)
ATGCCTGAATAGGGTGAAGCCAGAGTGGAATTCTCGGGTGCCAAGGAACT465770.15283553276343032RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAATGGAATTCTCGGGTGCCAAGGAA462020.1516050257581211RNA PCR Primer, Index 1 (100% over 23bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA458130.1503285798246137RNA PCR Primer, Index 1 (100% over 23bp)
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGTGCCAAGGAAC456440.14977403133422101RNA PCR Primer, Index 1 (100% over 24bp)
GCCAAGGACGTTTTCATTAATCAAGATGGAATTCTCGGGTGCCAAGGAAC452870.14860258866516668RNA PCR Primer, Index 1 (100% over 24bp)
TTTTGGTGGCAGGATAAATCCATAGGTGGAATTCTCGGGTGCCAAGGAAC446060.14636798794352518RNA PCR Primer, Index 1 (100% over 24bp)
TCGAAGAGTCGAGTTGTTTGGGAATGGAATTCTCGGGTGCCAAGGAACTC425600.1396543417225582RNA PCR Primer, Index 1 (100% over 26bp)
ATGTCCTAAGGGGGGCTCATGGAGAACATGGAATTCTCGGGTGCCAAGGA408580.13406948059446155RNA PCR Primer, Index 1 (100% over 22bp)
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTGCCAAGGAACT395410.12974793999181566RNA PCR Primer, Index 1 (100% over 25bp)
TTGGTGGCAGGATAAATCCATAGGATGGAATTCTCGGGTGCCAAGGAACT392740.12887181900403552RNA PCR Primer, Index 1 (100% over 25bp)
CCCACTAATAGGGAACGTGAGCTGGTGGAATTCTCGGGTGCCAAGGAACT382960.1256626567341891RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGTGGCAGGATAAATCCATAGGATGGAATTCTCGGGTGCCAAGGAAC376040.1233919611403919RNA PCR Primer, Index 1 (100% over 24bp)
AATGACCCACTCGGCACCTTACGAGATGGAATTCTCGGGTGCCAAGGAAC373180.12245349446434275RNA PCR Primer, Index 1 (100% over 24bp)
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCTCGGGTGCCAA372760.12231567767974812No Hit
TATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTC357790.1174034937145538RNA PCR Primer, Index 1 (100% over 26bp)
AGGTCCAGACACAATAAGGATTGACTGGAATTCTCGGGTGCCAAGGAACT345300.11330508504887066RNA PCR Primer, Index 1 (100% over 25bp)
ATGTCCTAAGGGGGGCTCATGGAGAACTGGAATTCTCGGGTGCCAAGGAA336140.11029936660390206RNA PCR Primer, Index 1 (100% over 23bp)
CCCACTAATAGGGAACGTGAGCTGGGTTGGAATTCTCGGGTGCCAAGGAA334380.10972184864941027RNA PCR Primer, Index 1 (100% over 23bp)
AAAATATGTACAGTAAAATTGTAAGTGGAATTCTCGGGTGCCAAGGAACT328920.10793023044968009RNA PCR Primer, Index 1 (100% over 25bp)
CAGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAAC310950.10203364088023842RNA PCR Primer, Index 1 (100% over 24bp)
AAATCTCCAGTAGAACAAAAGGGTAAATGGAATTCTCGGGTGCCAAGGAA309730.10163331593451116RNA PCR Primer, Index 1 (100% over 23bp)
GCCAAGGACGTTTTCATTAATCAAGTGGAATTCTCGGGTGCCAAGGAACT307300.10083594739507082RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)