FastQCFastQC Report
Thu 29 Jun 2023
SRR3312953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3312953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27976762
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT7987762.8551409916558605RNA PCR Primer, Index 1 (100% over 25bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG6496842.322227282771323Illumina Small RNA Adapter 2 (100% over 21bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG3670091.3118351580500989Illumina Small RNA Adapter 2 (100% over 21bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGA2578660.921714957578007RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC2469500.8826968610591891RNA PCR Primer, Index 1 (100% over 24bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA1708250.6105960368108361RNA PCR Primer, Index 1 (100% over 22bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGGGTGCCAAGGA1662530.5942539025781468RNA PCR Primer, Index 1 (100% over 22bp)
CCCACTAATAGGGAACGTGAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1607310.5745160930346407RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT1384120.49473916960082803RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC1026390.3668723349757202RNA PCR Primer, Index 1 (100% over 26bp)
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTGCCAAGGAACT1016550.3633551302327267RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGTGCCAAGGAAC1005480.35939827489685905RNA PCR Primer, Index 1 (100% over 24bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA959510.3429667807875693RNA PCR Primer, Index 1 (100% over 23bp)
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGTGCCAAGGAAC956970.34205888444130883RNA PCR Primer, Index 1 (100% over 24bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC868630.3104826784457758RNA PCR Primer, Index 1 (100% over 24bp)
GAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTC849740.30373064616984624RNA PCR Primer, Index 1 (100% over 26bp)
AGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAACT821460.293622256928804RNA PCR Primer, Index 1 (100% over 25bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGAA729800.2608593517720171RNA PCR Primer, Index 1 (100% over 23bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC719330.25711696013999047RNA PCR Primer, Index 1 (100% over 24bp)
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC704020.25164456129697926RNA PCR Primer, Index 1 (100% over 27bp)
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGGTGCCAAGGAA646510.23108821528381304RNA PCR Primer, Index 1 (100% over 23bp)
ATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAACT622040.22234167056216156RNA PCR Primer, Index 1 (100% over 25bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC595630.2129016931980906RNA PCR Primer, Index 1 (100% over 24bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT546710.19541575254491567RNA PCR Primer, Index 1 (100% over 25bp)
AAGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAA521660.18646189290955115RNA PCR Primer, Index 1 (100% over 23bp)
ATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACT517940.18513221794573653RNA PCR Primer, Index 1 (100% over 25bp)
AGGGTGAGAATCCCGTGTGGCGAGGATGGAATTCTCGGGTGCCAAGGAAC508180.1816436083632552RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGCGTCGCTGAACCATAGCAGTGGAATTCTCGGGTGCCAAGGAACTC505700.1807571583873788RNA PCR Primer, Index 1 (100% over 26bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA459980.1644150241546895RNA PCR Primer, Index 1 (100% over 23bp)
GAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAACT434880.1554432925440049RNA PCR Primer, Index 1 (100% over 25bp)
ATGTCCTAAGGGGGGCTCATGGAGAACATGGAATTCTCGGGTGCCAAGGA413830.1479191909342475RNA PCR Primer, Index 1 (100% over 22bp)
GAGGGTGAGAATCCCGTGTGGCGAGGATGGAATTCTCGGGTGCCAAGGAA387110.138368407323192RNA PCR Primer, Index 1 (100% over 23bp)
TCGAAGAGTCGAGTTGTTTGGGAATGGAATTCTCGGGTGCCAAGGAACTC375510.1342221090489314RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATAATGGAATTCTCGGGTGCCAAGGAA371210.13268511917140374RNA PCR Primer, Index 1 (100% over 23bp)
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCTCGGGTGCCAA366520.1310087278863794No Hit
AGGGTGAGAATCCCGTGTGGCGAGGAGTGGAATTCTCGGGTGCCAAGGAA360570.12888196282328884RNA PCR Primer, Index 1 (100% over 23bp)
TCGAAGAGTCGAGTTGTTTGGGAATTGGAATTCTCGGGTGCCAAGGAACT350430.12525752622837483RNA PCR Primer, Index 1 (100% over 25bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTGGAATTCTCGGGTGCCAAGGA349970.12509310405543RNA PCR Primer, Index 1 (100% over 22bp)
GCCAAGGACGTTTTCATTAATCAAGATGGAATTCTCGGGTGCCAAGGAAC345290.12342028716546968RNA PCR Primer, Index 1 (100% over 24bp)
GATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAAC344560.12315935632579639RNA PCR Primer, Index 1 (100% over 24bp)
TGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAA336460.12026409632394199RNA PCR Primer, Index 1 (100% over 23bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA317700.11355853118384465RNA PCR Primer, Index 1 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC316570.11315462454161065No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTGGAATTCTCGGGTGCCAAGGAA312860.11182852397286004RNA PCR Primer, Index 1 (100% over 23bp)
CAGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAAC312500.11169984575055542RNA PCR Primer, Index 1 (100% over 24bp)
AATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAAC298270.1066134815744581RNA PCR Primer, Index 1 (100% over 24bp)
GCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAGGA291560.10421506248650218RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)