FastQCFastQC Report
Wed 28 Jun 2023
SRR3312954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3312954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34993471
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT10612943.0328343250087997RNA PCR Primer, Index 1 (100% over 25bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG5852591.6724805607308861Illumina Small RNA Adapter 2 (100% over 21bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG4350181.2431404704037505Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT2191010.6261196552922687RNA PCR Primer, Index 1 (100% over 25bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA2125170.6073047169284808RNA PCR Primer, Index 1 (100% over 22bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGA2005510.5731097666761894RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC1998830.5712008391508233RNA PCR Primer, Index 1 (100% over 24bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC1939330.5541976673305715RNA PCR Primer, Index 1 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC1592400.4550563160767905RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGTGCCAAGGAAC1549380.442762594199358RNA PCR Primer, Index 1 (100% over 24bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTGGAATTCTCGGGTGCCAAGGA1466650.41912104117936744RNA PCR Primer, Index 1 (100% over 22bp)
GAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTC1373660.3925475126488596RNA PCR Primer, Index 1 (100% over 26bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC1332500.38078531849555597RNA PCR Primer, Index 1 (100% over 24bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA1326630.37910786272102015RNA PCR Primer, Index 1 (100% over 23bp)
CCCACTAATAGGGAACGTGAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1288720.368274413246974RNA PCR Primer, Index 1 (100% over 27bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC932870.2665840150581233RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAACT924680.26424357846639446RNA PCR Primer, Index 1 (100% over 25bp)
AGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAACT913840.2611458577515789RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCC912910.2608800938895144No Hit
ATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACT911310.2604228657397261RNA PCR Primer, Index 1 (100% over 25bp)
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC882820.2522813469975585RNA PCR Primer, Index 1 (100% over 27bp)
AAGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAA723550.2067671423620709RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGCGTCGCTGAACCATAGCAGTGGAATTCTCGGGTGCCAAGGAACTC673530.19247304732931467RNA PCR Primer, Index 1 (100% over 26bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGAA662510.18932388844764783RNA PCR Primer, Index 1 (100% over 23bp)
AGGGTGAGAATCCCGTGTGGCGAGGATGGAATTCTCGGGTGCCAAGGAAC646030.18461443850482853RNA PCR Primer, Index 1 (100% over 24bp)
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGGTGCCAAGGAA642800.18369140917744342RNA PCR Primer, Index 1 (100% over 23bp)
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGTGCCAAGGAAC599240.17124337279945737RNA PCR Primer, Index 1 (100% over 24bp)
AATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAAC587120.1677798695648111RNA PCR Primer, Index 1 (100% over 24bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT582080.166339600892978RNA PCR Primer, Index 1 (100% over 25bp)
ATGTCCTAAGGGGGGCTCATGGAGAACATGGAATTCTCGGGTGCCAAGGA571140.1632133034188006RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAATGGAATTCTCGGGTGCCAAGGAA568190.16237028901762846RNA PCR Primer, Index 1 (100% over 23bp)
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTGCCAAGGAACT546920.15629201230138043RNA PCR Primer, Index 1 (100% over 25bp)
TCGAAGAGTCGAGTTGTTTGGGAATGGAATTCTCGGGTGCCAAGGAACTC523820.14969078088881208RNA PCR Primer, Index 1 (100% over 26bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA499960.1428723661050943RNA PCR Primer, Index 1 (100% over 23bp)
GATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAAC491960.14058622535615287RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGTGAGAATCCCGTGTGGCGAGGATGGAATTCTCGGGTGCCAAGGAA486710.13908594548966005RNA PCR Primer, Index 1 (100% over 23bp)
TATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACTC485080.13862014431206326RNA PCR Primer, Index 1 (100% over 26bp)
TCGAAGAGTCGAGTTGTTTGGGAATTGGAATTCTCGGGTGCCAAGGAACT471550.13475370877041606RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGTGGCAGGATAAATCCATAGGTGGAATTCTCGGGTGCCAAGGAACT437270.12495759566120207RNA PCR Primer, Index 1 (100% over 25bp)
GAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAACT433960.12401170492632756RNA PCR Primer, Index 1 (100% over 25bp)
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCTCGGGTGCCAA433180.12378880620330575No Hit
TTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAACTC431380.12327442453479392RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGGTGCCAAGGAA402270.11495572988458333RNA PCR Primer, Index 1 (100% over 23bp)
AGGGTGAGAATCCCGTGTGGCGAGGAGTGGAATTCTCGGGTGCCAAGGAA400060.11432418350268826RNA PCR Primer, Index 1 (100% over 23bp)
GCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAGGA394350.11269245054313132RNA PCR Primer, Index 1 (100% over 22bp)
AGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAA393190.11236096013453481RNA PCR Primer, Index 1 (100% over 23bp)
GCCAGCTGGGACTGAGGACTGCGACGTGGAATTCTCGGGTGCCAAGGAAC386260.1103805907107643RNA PCR Primer, Index 1 (100% over 24bp)
GCCAAGGACGTTTTCATTAATCAAGATGGAATTCTCGGGTGCCAAGGAAC378720.10822590305488702RNA PCR Primer, Index 1 (100% over 24bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTGGAATTCTCGGGTGCCAAGGA376230.10751434174677899RNA PCR Primer, Index 1 (100% over 22bp)
ATGATTAATAGGGACGGTCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT367460.10500815995075197RNA PCR Primer, Index 1 (100% over 25bp)
TTTTGGTGGCAGGATAAATCCATAGGTGGAATTCTCGGGTGCCAAGGAAC365650.10449092060630395RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGGCAGGATAAATCCATAGGTGGAATTCTCGGGTGCCAAGGAACTC361310.10325068925000323RNA PCR Primer, Index 1 (100% over 26bp)
CAGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAAC350760.10023584113733673RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)