FastQCFastQC Report
Sun 2 Jul 2023
SRR3328250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3328250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31097531
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT5873621.888773742198376No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC3687711.1858529862065255No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG2595070.8344939024258872No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC2229020.7167835928839494No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA1977780.6359926130469972No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1811890.5826475420186895No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1749310.5625237579150576Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAAG1650170.5306434134594158No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1570260.5049468396703263No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1368870.44018607136367194No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA1132400.36414466473238666No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1118040.35952693479106107No Hit
TTAATCAAACTCCGAATACCATTACTGTAGGCACCATCAATAGATCGGAA1116000.3588709341587279No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1097760.3530055167402196No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA1086660.3494361015348775No Hit
CCCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCG1025710.3298364747992373No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG989430.3181699537497044No Hit
CCCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGG966610.3108317505978208No Hit
CCTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGA955410.30723017849873674No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT924340.2972390316131528No Hit
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA920290.295936677416609No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC907960.2919717324182424No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG896320.28822866998669444No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA885150.284636744955733No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA877470.2821670955163611No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA867460.2789481904528047No Hit
CCTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGG822310.26442935292837233No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA758810.24400972540231572No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG748380.24065576138504371No Hit
CTCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAA734810.23629207090427856No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA715360.23003755507149426No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT713790.22953269183974764No Hit
ATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAA709170.2280470433488755No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC659690.21213581232542222No Hit
TGAATGATTTCATGTTTCCAAGAAAAGCTTCTGTAGGCACCATCAATAGA641200.20619000267255946No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA639030.20549219807836192No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC631840.20318011741832495No Hit
CCCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGG613900.19741117068104216No Hit
CTCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGA572760.18418182459565682No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA566420.18214307753242534No Hit
CCGCGGACGCTACGCCGCGACGAGTCTGTAGGCACCATCAATAGATCGGA554980.17846432888836095No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC540040.17366008896333282No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA537820.17294620592226437No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG537300.1727789900748069No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAA536080.17238667597115667No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGATCGG532870.17135443968204422No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC523010.16818376995910062No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC521750.16777859309795365No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA509130.16372039310773578No Hit
TCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAG495080.15920234953700985No Hit
ATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAAG487370.15672305302951545No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCA479560.15421159962827916No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA475660.15295748077234814No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGCTGTAGGCACCATCAATAGATC463930.1491854771364325No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG459930.1478992013867596No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT458540.1474522205637483No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG450230.1447799826938029No Hit
TCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAA445390.14322358903669877No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA426870.13726813231571341No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG407030.13088820459733605No Hit
ACGAATTGAATGATTTCATGTTTCCAAGAAAAGCTTCTGTAGGCACCATC401340.1290584773434264No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG397770.12791047623684337No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAAT388910.12506137545131799No Hit
GCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGGA381890.12280396151064212No Hit
TCTTGAGTTGTGATGCATAGTGACTGTAGGCACCATCAATAGATCGGAAG376820.12117360699793177No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCCTGTAGGCACCAT376140.12095494012048738No Hit
CTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATCTGTAGGCACCATC375710.12081666547739754No Hit
AGGATAGCTGGCGCTCTCGCAGACCCTGTAGGCACCATCAATAGATCGGA369450.11880364392915953No Hit
ACGTGGGGATGGAACACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA366450.11783893711690488No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC360940.11606709227173051No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG358570.11530497389004934No Hit
AGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATAGATCGG348410.11203783348588028No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA346310.11136253871730202No Hit
GCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAGA342480.11013092968699026No Hit
ACGATGATCATTAGTTGGTGGAATCTGTAGGCACCATCAATAGATCGGAA326540.10500512082454391No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA324430.10432661036659148No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCTGTAGGCACCATCAATAGATCGGA323750.1041079434891471No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCTGTAGGCACCAT320360.10301782479129934No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG313250.10073146964625584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)