FastQCFastQC Report
Tue 26 Sept 2023
SRR3356481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46024962
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA22082864.798018084186577No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC8863611.925826685093189No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG8686721.8873931932849832No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA6667191.448603042844446No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC5449331.1839944593544693No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG4878791.060031293453322No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA2732220.5936387302177457No Hit
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA2362390.5132845085238745RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA2072450.45028825879313056RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA1975790.42928661190420975RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1658850.36042398036091805No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGACTTCTCGGGTGCCA1630080.3541730246295478No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1616900.35130936121142264Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA1532980.33307577744442246RNA PCR Primer, Index 1 (100% over 23bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG1414560.30734626136138904No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA1381110.3000784661158438No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT1348900.2930800898868749RNA PCR Primer, Index 1 (100% over 25bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1210870.26308984242072814RNA PCR Primer, Index 1 (100% over 33bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1132850.24613817171646987Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG1102250.2394896056622491Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC889990.19337115367960542RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA847860.18421742531802635No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA773260.16800882964335745RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAAG755790.16421306333723862No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA750850.16313973273894283No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGCATTCTCGGGTGCC729020.1583966544067978No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC722510.15698220456977238No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTTGGAATTCTCGGGTGCCAA716010.15556992746675163No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAGG707190.15365357607465271Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC672360.14608594353646615RNA PCR Primer, Index 1 (100% over 24bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA662290.1438980003937863No Hit
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC631070.13711472483127743RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG575640.12507126024351742No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAA573440.12459325876249501No Hit
AAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGCCAAGGAA571410.12415219375955162RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA562020.1221119965291878RNA PCR Primer, Index 1 (100% over 23bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG552540.12005224469278214Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA527190.11454436399100122RNA PCR Primer, Index 1 (100% over 23bp)
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG508440.11047048773228753Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGTGGCTGGTTGAGATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG487630.1059490282686165RNA PCR Primer, Index 1 (100% over 29bp)
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGGTGGAATTCTCGGGTGCCA473610.10290285519410097No Hit
CGGACGTTGACTGTCACTGATTTCTGCCCATGGAATTCTCGGGTGCCAAG468360.10176216984166114No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA462790.1005519570010726RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)