FastQCFastQC Report
Tue 26 Sept 2023
SRR3356482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44542999
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA6440551.4459174605643415RNA PCR Primer, Index 4 (100% over 40bp)
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT5179181.1627371565170095RNA PCR Primer, Index 1 (100% over 25bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC5078851.1402128536518163RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA4567881.0254989790876003RNA PCR Primer, Index 1 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC2932270.658300982383337RNA PCR Primer, Index 1 (100% over 24bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG2691980.6043553555969593No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC2607540.585398392236679No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA2367890.5315964468400523No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA2312230.5191006559751399No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA2182540.4899849693551168RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA2131530.47853311358761447RNA PCR Primer, Index 4 (100% over 40bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG2089360.4690658570160487No Hit
AAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA2065850.4637878109644122RNA PCR Primer, Index 4 (100% over 39bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG1994470.4477628459637394No Hit
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1835370.4120445504803123RNA PCR Primer, Index 4 (100% over 37bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA1719760.38608985443481253RNA PCR Primer, Index 1 (100% over 23bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA1623300.3644343749732702No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA1594040.357865441435589RNA PCR Primer, Index 1 (100% over 23bp)
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA1477100.33161215750201284RNA PCR Primer, Index 1 (100% over 23bp)
AAGGCGGGGTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC1445470.32451115381790974RNA PCR Primer, Index 4 (100% over 38bp)
GTGGGTTCGTGGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1214560.27267135740007087RNA PCR Primer, Index 1 (100% over 33bp)
CAATGAGTTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1211760.27204275131991RNA PCR Primer, Index 4 (100% over 37bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC1165430.2616415657149623No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1093470.24548638945482767RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA1091500.2450441201770002RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC942240.21153492606099558RNA PCR Primer, Index 1 (100% over 33bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG939420.21090182993740497No Hit
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC861130.19332555493176382RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA826240.18549267416861626RNA PCR Primer, Index 4 (100% over 36bp)
CTGTGGTCGTGACTTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC726680.16314123797546726RNA PCR Primer, Index 1 (100% over 33bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG723200.16235997041869588Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG696620.15639270270059724Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT684710.15371888183819865RNA PCR Primer, Index 4 (100% over 41bp)
CGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC676050.15177469303312963RNA PCR Primer, Index 1 (100% over 31bp)
CTTGGTCAAGGTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC549990.12347394929560086RNA PCR Primer, Index 1 (100% over 33bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA535450.120209687722194RNA PCR Primer, Index 1 (100% over 22bp)
CAATGAGTTGAATACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG530840.11917473271164342RNA PCR Primer, Index 4 (100% over 35bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCCAGGAAC500450.112352111720183RNA PCR Primer, Index 1 (95% over 24bp)
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC491500.11034281728538305RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCTGGAATTCTCGGGTGCCAAGGAACTC482450.10831107263343448RNA PCR Primer, Index 1 (100% over 26bp)
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT473790.10636688382836548RNA PCR Primer, Index 1 (100% over 25bp)
TTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTC472010.10596726996312035RNA PCR Primer, Index 1 (100% over 26bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC471790.10591787948539343No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)