FastQCFastQC Report
Tue 26 Sept 2023
SRR3356487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49229897
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA9990212.029297359691815RNA PCR Primer, Index 1 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC8198871.6654249753965562RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT6075521.2341118649913079RNA PCR Primer, Index 1 (100% over 25bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC5434671.1039369024070882No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC4861320.9874731202464225RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG3074150.6244477822084413No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA2819900.5728023359463864RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG2530650.5140473887239699No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA2301200.4674395317138283RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA2281370.4634114915982863RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA2278410.4628102309456386RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2227780.4525258299849785No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA2039500.41428077739021063No Hit
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA1810440.36775214053362737RNA PCR Primer, Index 1 (100% over 23bp)
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA1599440.3248920061725906RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1409940.2863991366872045RNA PCR Primer, Index 1 (100% over 33bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA1315610.2672380159560358No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC1060770.21547272382064905RNA PCR Primer, Index 1 (100% over 24bp)
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT1015150.20620599714031496RNA PCR Primer, Index 1 (100% over 25bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG986990.20048589579620696Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAAC892610.18131461863509485RNA PCR Primer, Index 1 (100% over 24bp)
TACGCCGACGGCGTTGGCTGCTCGTTCTAGTGGAATTCTCGGGTGCCAAG864680.17564123686872635No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG824580.16749578005414067Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC824030.16738405932476358No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA747090.15175534492790022No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG602890.12246420097121065Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG602050.12229357294816197Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA599160.12170653129743499RNA PCR Primer, Index 1 (100% over 22bp)
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA597670.12140386968512244RNA PCR Primer, Index 4 (100% over 40bp)
AGCAGTGGCTGGTTGAGATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG580550.11792630807251131RNA PCR Primer, Index 1 (100% over 29bp)
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC579980.11781052477115685RNA PCR Primer, Index 4 (100% over 37bp)
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC524940.10663032668949113RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)