FastQCFastQC Report
Tue 26 Sept 2023
SRR3356488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA18587253.71745RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA13640152.72803No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC12117442.423488No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC6115181.223036No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC5504281.100856RNA PCR Primer, Index 1 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA4176430.835286RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA4116490.8232980000000001RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT3404780.680956RNA PCR Primer, Index 1 (100% over 25bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC3232410.646482RNA PCR Primer, Index 1 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA2934670.586934RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG2822650.56453No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA2405860.48117200000000004No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG1902950.38059No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG1806330.36126600000000003Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC1569760.313952RNA PCR Primer, Index 1 (100% over 24bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA1488860.29777200000000004RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG1387820.277564Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG1238280.24765600000000002No Hit
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT1000140.200028RNA PCR Primer, Index 1 (100% over 25bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA865300.17306000000000002RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC748900.14978RNA PCR Primer, Index 1 (100% over 24bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA713110.142622No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA698310.13966199999999998No Hit
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA691870.138374RNA PCR Primer, Index 6 (100% over 37bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG660160.132032Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC659310.131862RNA PCR Primer, Index 1 (100% over 33bp)
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA652710.13054200000000002RNA PCR Primer, Index 1 (100% over 23bp)
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA641520.128304RNA PCR Primer, Index 6 (100% over 40bp)
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA539860.107972No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG519230.103846Illumina Small RNA Adapter 2 (100% over 21bp)
CTAATTTGCTGGTTTGAGGTTGGGTTGGAATTCTCGGGTGCCAAGGAACT515470.10309399999999999RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)