FastQCFastQC Report
Tue 26 Sept 2023
SRR3356489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800300
Total Bases91.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA629993.4993612175748487RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA455952.5326334499805587No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC416832.3153363328334167No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC211301.1736932733433316No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC184311.0237738154751985RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA145960.8107537632616787RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA143160.7952007998666889RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT121060.6724434816419486RNA PCR Primer, Index 1 (100% over 25bp)
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC113230.6289507304338166RNA PCR Primer, Index 1 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA97960.5441315336332834RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG92490.5137477087152141No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA81760.45414653113369996No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG65940.366272287952008No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG62230.34566461145364663Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC53970.2997833694384269RNA PCR Primer, Index 1 (100% over 24bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG51110.2838971282564017Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA50100.27828695217463756RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG41770.23201688607454313No Hit
TGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAACT33170.18424706993278897RNA PCR Primer, Index 1 (100% over 25bp)
CTAAATCACTTGCATACGACTTGGTCTTGGAATTCTCGGGTGCCAAGGAA31070.17258234738654668RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAAC26220.14564239293451092RNA PCR Primer, Index 1 (100% over 24bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA24060.13364439260123315No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGGAATTCTCGGGTGCCAA23430.13014497583736045No Hit
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA23300.1294228739654502RNA PCR Primer, Index 6 (100% over 37bp)
TCGACTCTTTACTTATTCGGTTAAAAGTGGAATTCTCGGGTGCCAAGGAA23080.12820085541298673RNA PCR Primer, Index 1 (100% over 23bp)
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA21130.11736932733433317RNA PCR Primer, Index 6 (100% over 40bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG20960.11642504027106593Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGGCTGGTTGAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC19950.1108148641893018RNA PCR Primer, Index 1 (100% over 33bp)
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA18880.10487141032050214No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG18070.10037216019552297Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)