FastQCFastQC Report
Tue 26 Sept 2023
SRR3356492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62890589
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC35632735.665828634551348No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA28909144.596735451149933No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA27359544.350339285262537RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG19248033.0605580749132435Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC6803361.08177711612782No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC6234800.9913724929496208No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG6080330.9668107894489587No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA6068710.9649631362174076RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA5846410.9296160352385952No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC5706170.9073169914182232No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG5565630.8849702457071916Illumina Small RNA Adapter 2 (100% over 21bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG5315490.845196409275162Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA4604320.7321158973403794RNA PCR Primer, Index 1 (100% over 23bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA4144060.6589316566903197No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG3640330.5788354120836744No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC3432830.5458416043774054No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA3294980.5239225856192887No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC3213800.5110144540067831No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC2840170.45160492931621293No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG2827410.44957600890015514No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2759910.4388430835017303No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCGGGTGCCA2604530.4141366842660672No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2333070.37097283347115734Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG2195020.349022013452601Illumina Small RNA Adapter 2 (100% over 21bp)
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC2161420.34367940169871836No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCGGGTGCC2023260.3217110909869202No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1995680.3173256971722748No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA1849780.2941266776814572No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC1832320.29135042764506464No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA1778310.28276249726330277RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG1733840.2756914870045183Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1652820.2628087964003645No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAG1637290.2603394285272157No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1605300.25525281691987334No Hit
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA1599080.25426379772019625No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1548660.24624669996332837RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA1532830.24372963019952RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG1527930.24295049931874543Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA1467640.23336400935917456No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1463920.23277250591499468No Hit
ATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGC1457570.23176281589603176No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1324880.21066426965726143No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1317050.20941925031104416RNA PCR Primer, Index 1 (100% over 23bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG1273770.20253745755187633No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG1264630.20108413994977847Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1247140.19830311972431997No Hit
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1157220.18400527303059605No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC1117800.1777372445979159No Hit
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGTGGAATTCTCGGG1111100.17667190237318336No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTTGGAATTCTCGGGTGC1097910.17457460924718005No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1084960.17251547763370445No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1060430.16861505304076577No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG1058970.1683829038395554No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1036520.1648132123551904Illumina Small RNA Adapter 2 (100% over 21bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG983870.15644153054441898Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG943950.1500939989606394Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG933530.148437153291727Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA921550.14653225779138435No Hit
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA907320.1442695981110942RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCCGGTGCCA885660.1408255216054663No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA854700.13590268648938875No Hit
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA845850.1344954807149286No Hit
CATCCGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG815160.12961557730044473Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCAA798180.12691565028910765No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG792560.1260220348707499No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCCGGTGCC780830.12415689094595694No Hit
TCCTTAGGTCACTGGTTCGAATCCGGTTCGACGGTGGAATTCTCGGGTGC769540.12236170979413152No Hit
GCGTTCGTGGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG767360.12201507605533796No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA760500.12092429282225359RNA PCR Primer, Index 1 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG749310.11914501230064804Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGCGGCCCGGGTTCGATTCCCGGTCGATGCTGGAATTCTCGGGTGC719240.1143636927935275No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA716040.11385487262649108RNA PCR Primer, Index 1 (100% over 23bp)
AGGAAGGTCGTGGCGGTTGCGTTGAAGGCTTGGAATTCTCGGGTGCCAAG714980.11368632594616025No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCA713460.11344463636681794No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA707050.11242540596972307No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACTGGAATTCTCGGGTGCCA692830.1101643363524549No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA690340.10976841065997968No Hit
ATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGGA687660.10934227377008665RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC677030.10765203677771248No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG667320.10610808558336128No Hit
AACTATGCCTGAGCAGGATGAAGCCAGAGGTGGAATTCTCGGGTGCCAAG640800.1018912384490468No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG640530.10184830674745311Illumina Small RNA Adapter 2 (100% over 21bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCGTGGAATTCTCGGGTGCCAAGG637440.10135697727365854Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)