FastQCFastQC Report
Tue 26 Sept 2023
SRR3356493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65028207
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG31220564.8010796299519685Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC29946504.6051554212466606No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA25649443.9443560238405464No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA21980943.38021621909397RNA PCR Primer, Index 1 (100% over 22bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG11879521.8268257034981756No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA8394701.2909321027411995RNA PCR Primer, Index 1 (100% over 23bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC7688161.1822807908574198No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC7301651.1228435069722897No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG6910751.062731131430396Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC6819931.0487648844446842No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA6131520.9429015934577437No Hit
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG5747890.8839071943041579Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG5119710.7873060378244782No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA4616900.7099842073148349RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC3799690.5843141269449426No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG3710500.570598540414931No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA3709690.5704739790841842No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC3278330.5041396881817763No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC3063400.47108787729607865No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG2991130.4599742385638897No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC2865620.4406733834749588No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA2803340.43109600115531405No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2798330.43032556625773183Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG2686610.4131453293799105Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2673550.4111369701458938No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC2446930.37628747783250427No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG2207360.3394465420213723No Hit
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA2101890.3232274265227703No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCGGGTGCC1894910.29139816203143964No Hit
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1866110.286969314715997No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCGGGTGCCA1855090.2852746655001575No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA1817390.2794771813407065No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG1691960.26018862860542963No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA1686140.2592936323771006No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1685460.2591890623710415No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA1684790.25908603015918924No Hit
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC1677010.257889626266337No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1665090.25605657557188993No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAG1652350.2540974257524892No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA1644100.2528287455319197RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG1538390.23657272297235568Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGC1491420.22934970358324658No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA1404250.21594475148299874No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG1392460.21413169211323943Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGTGGAATTCTCGGG1365040.20991506039832836No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1362200.20947832684361112No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1298020.19960876362468366No Hit
ACAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCA1221050.1877723616153218No Hit
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1220740.1877246899949125No Hit
GCGTTCGTGGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG1192660.1834065638623559No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG1171180.1801033819062549No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG1154490.17753680337518762Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA1106330.17013078647547517RNA PCR Primer, Index 1 (100% over 23bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG1105730.1700385188230701Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG1105440.16999392279107434Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG1069160.16441480540898198Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC1033100.15886951949943814No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTTGGAATTCTCGGGTGC1011760.15558786666223168No Hit
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA1010820.15544331400679706No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1000030.15378403405771282No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA983570.15125282479340083RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGCGGCCCGGGTTCGATTCCCGGTCGATGCTGGAATTCTCGGGTGC980720.1508145534444768No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG971260.14935980012489042Illumina Small RNA Adapter 2 (100% over 21bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG959920.14761594149443488Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCAA929210.14289337548550277No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG927550.14263810164718213No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCGTGGAATTCTCGGGTGCCAAGG919480.14139710172233413Illumina Small RNA Adapter 2 (100% over 21bp)
CATCCGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG910170.13996541531584902Illumina Small RNA Adapter 2 (100% over 21bp)
CACCCGGTCCGGACACCATTAGGACTGACAGATGGAATTCTCGGGTGCCA905080.1391826780646128No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA886450.13631776745743582No Hit
AGGAAGGTCGTGGCGGTTGCGTTGAAGGCTTGGAATTCTCGGGTGCCAAG882650.13573340565887046No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA864880.13300074535347406No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC853690.13127995363611977No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAA850130.13073249889851646No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCCGGTGCC844270.12983135149336042No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCGCTGGAATTCTCGGGTGCCAAGG836870.12869338378036474Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACTGGAATTCTCGGGTGCCA834860.1283842871448078No Hit
GCGTTCGTCGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG816650.12558396389431437No Hit
AACTATGCCTGAGCAGGATGAAGCCAGAGGTGGAATTCTCGGGTGCCAAG811230.12475047943425535No Hit
CGTTCGTGGTGTAATGGTCAGCATGGATGCTGGAATTCTCGGGTGCCAAG762300.11722605238062306No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA758060.11657402763696069No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCC740620.11389211454038706No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG738260.11352919510759385Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCCGGTGCCA727570.1118852931005771No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCC723160.11120712585539995No Hit
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA709250.1090680541138094RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA694270.10676443839209653RNA PCR Primer, Index 1 (100% over 23bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCA693350.10662296132507544No Hit
TTCTCTAGTTAAGGAACGGACTCCCTGGAATTGGAATTCTCGGGTGCCAA660470.10156669397327839No Hit
TCCTTAGGTCACTGGTTCGAATCCGGTTCGACGGTGGAATTCTCGGGTGC658000.10118685880421091No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA656160.10090390467016874RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)