FastQCFastQC Report
Tue 26 Sept 2023
SRR3356495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56796840
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA43934417.735361685614904RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG38589126.794237144179148Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG14899252.6232533359250265No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA9588751.6882541352652718No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC8848411.5579053341699995No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG7357491.2954048147749067Illumina Small RNA Adapter 2 (100% over 21bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC5670310.9983495560668515No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA5540770.9755419491647774No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC5458230.9610094505257688No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG5261110.9263032943382061No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA5041610.8876567780883584RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA4804270.845869242021211RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG4004110.7049881648345224No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA3911840.6887425427189259RNA PCR Primer, Index 1 (100% over 23bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG3275840.5767644819676587Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC3085250.5432080376302625No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG3071580.5408012135886433No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2750130.48420475505327415RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA2491220.43861947249177946RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA2414710.42514865263630863RNA PCR Primer, Index 1 (100% over 23bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG2394750.4216343726165047No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2333980.4109348336984945Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2298170.4046299054665717No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC2082620.366678850443088No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG1959450.3449927848098592Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1916180.3373744032238413RNA PCR Primer, Index 1 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG1810540.31877477690660255Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA1775990.31269169200258323No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA1758050.30953306557195787RNA PCR Primer, Index 1 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1741030.30653641998392867RNA PCR Primer, Index 1 (100% over 22bp)
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1595800.2809663354510568No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1558100.2743286422272789No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA1503020.2646309196074993RNA PCR Primer, Index 1 (100% over 23bp)
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1455540.25627129960047074No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA1448070.2549560855850431No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1414980.24913005723557863No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1391000.24490799136008273No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC1381720.24327409764346042No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1353660.23833368194427718No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG1353190.23825093086164653Illumina Small RNA Adapter 2 (100% over 21bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1331400.23441444981798285Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1276960.22482940952348757RNA PCR Primer, Index 1 (100% over 23bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA1264990.22272189790840477No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA1258960.22166021912486683No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA1240600.21842764491827363No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG1215950.21408761473349575Illumina Small RNA Adapter 2 (100% over 21bp)
TTCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG1179270.20762950896563967No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG1175280.20692700509394538Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA1023160.18014382490293473No Hit
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1011590.1781067397411546No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA1009280.17770002697333162No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG1006350.17718415320288947No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC992830.17480373908125874No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG979930.1725324859622472No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG968620.1705411779951138Illumina Small RNA Adapter 2 (100% over 21bp)
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA951360.16750227653510302No Hit
ACAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCA945070.16639482055691832No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC937700.16509721315481635RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC878650.15470050798600765No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG874030.15388708245036167Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCGGTAGTATAGTGGTGAGTATCCGCTGGAATTCTCGGGTGCCAAGG840790.14803464418090864Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG828750.1459148079364979No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG821900.14470875492368943Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAA763120.13435958761086003No Hit
ATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGC761050.13399513071501867No Hit
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA743970.13098792115899405RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA740420.13036288638593274RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC737430.12983644864749516No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA720560.1268662129794545No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAG719880.12674648800883995No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA693690.1221353159788467RNA PCR Primer, Index 1 (100% over 22bp)
AACTATGCCTGAGCAGGATGAAGCCAGAGGTGGAATTCTCGGGTGCCAAG692620.12194692521626203No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG684220.12046796969690567Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGGA665930.11724772011964045RNA PCR Primer, Index 1 (100% over 22bp)
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA638370.11239533748708555RNA PCR Primer, Index 1 (100% over 22bp)
TTCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA619700.1091081827791828No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAA615130.10830356055019962No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA612270.10780001140908543No Hit
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA612180.10778416545709232RNA PCR Primer, Index 1 (100% over 23bp)
GCGTTCGTGGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG600230.10568017516467466No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA598630.10539846935146392No Hit
TAGTCGATAGAATAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAGGA597980.10528402636484707RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCCGGTGCCAAGG590610.1039864189627451Illumina Small RNA Adapter 2 (95% over 21bp)
ACTATGCCTGAGCAGGATGAAGCCAGAGGTGGAATTCTCGGGTGCCAAGG588360.10359027016291751Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTCTAGTTAAGGAACGGACTCCCTGGAATTGGAATTCTCGGGTGCCAA585990.10317299342709912No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACTGGAATTCTCGGGTGCCA582810.10261310312334278No Hit
ATCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGA574530.10115527553997722RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)