FastQCFastQC Report
Tue 26 Sept 2023
SRR3356498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68765583
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA46004446.69003853279336RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG39113045.687880229271087Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG16827202.447038077172995No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA7718501.122436495594024No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG6576900.9564232153750518Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA6417660.9332662823494131RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA6366640.9258468731371041RNA PCR Primer, Index 1 (100% over 22bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC5933350.8628371550343723No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA5587510.8125445544466626RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG5176860.7528271810041951No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC5062880.7362520288674059No Hit
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG4758120.691933346947702Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA4509490.6557771785342095RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC4233420.61563064185757No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA3850720.5599778016860557No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG3041910.4423593703844553No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA2908100.42290050823825637RNA PCR Primer, Index 1 (100% over 23bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC2896810.4212586985556423No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2856520.41539966293894437RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2750160.3999326232717318Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG2560090.37229234281341No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG2273090.33055634822437263No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA2180740.3171266649480744RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2070000.3010226787432312No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1977610.2875871786035756No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1913460.2782583839942141RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1905990.27717208476222766RNA PCR Primer, Index 1 (100% over 22bp)
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA1816910.26421793006539335No Hit
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA1678460.244084311769741RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1662130.24170957730409995No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG1596910.23222518159992916Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA1572450.22866816965690526No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG1553030.2258440824969084Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1521950.2213243796682419No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1502210.21845375760138613No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG1500100.21814691805928554Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA1495210.21743580651384867RNA PCR Primer, Index 1 (100% over 22bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1487670.2163393277709868Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG1466290.21323021430647943Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA1454430.21150551432102307No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1401660.20383161733682967No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1343220.19533317997173094No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA1304590.1897155441843633No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1296190.18849400287931828No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA1257130.18281383581085905No Hit
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1201930.1747865643777062RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC1186460.17253689247424833No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1158170.16842291586475752No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC1067810.15528262154048778No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1040510.15131261229909154Illumina Small RNA Adapter 2 (100% over 21bp)
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA1040210.15126898582391138No Hit
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA1038390.15100431854115162RNA PCR Primer, Index 1 (100% over 23bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG1009890.14685980339903465No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA992940.1443949075513546RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG985230.143273707139224Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG972230.1413832265480829Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG949290.13804725541263863No Hit
ACAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCA948910.13799199521074373No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA941890.13697113569152755No Hit
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC939640.1366439371276762No Hit
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA934210.13585429792691497RNA PCR Primer, Index 1 (100% over 22bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA925060.13452369043391954No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG901790.13113973017577701No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCCGGTGCCAAGGA887740.12909655692150535RNA PCR Primer, Index 1 (95% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCCGGTGCCAAGG883570.12849014891650085Illumina Small RNA Adapter 2 (95% over 21bp)
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG879740.12793318425003392No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCAA868400.12628410348822317No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA866150.12595690492437184No Hit
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA863880.12562679792884182RNA PCR Primer, Index 1 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC834620.12137176238293508RNA PCR Primer, Index 1 (100% over 24bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG820320.1192922337326799Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAG819120.11911772783195919No Hit
AACTATGCCTGAGCAGGATGAAGCCAGAGGTGGAATTCTCGGGTGCCAAG813480.11829755009857183No Hit
CATCCGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG801640.11657575854479413Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCGGTAGTATAGTGGTGAGTATCCGCTGGAATTCTCGGGTGCCAAGG800890.11646669235684368Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCCGGTGCCA795240.115645060407617No Hit
TTCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG794210.11549527617616503No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGA790620.11497321268984224RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA755620.10988345725215477RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAA736870.1071568025533936No Hit
ATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGGA725970.10557170728851378RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC721720.10495366555679461RNA PCR Primer, Index 1 (100% over 24bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG708880.10308645241908297Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGCGGCCCGGGTTCGATTCCCGGCATGGGATGGAATTCTCGGGTGCC707250.10284941523727065No Hit
TCTGTTGAGACTATCCTTTGATTGAGTTTGGAATTCTCGGGTGCCAAGGA699690.10175002806273015RNA PCR Primer, Index 1 (100% over 22bp)
AATCGATCGATAGACTTGGGTATAGGGTGGAATTCTCGGGTGCCAAGGAA693620.10086731904825122RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC687970.10004568709902452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)