FastQCFastQC Report
Tue 26 Sept 2023
SRR3356503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59706751
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA49295518.256270718867285RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG22505783.7693861452953623Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA9077181.520293743667278RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA9006411.508440812664551RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG6617331.1083051563130608Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC6035301.0108237174050887No Hit
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG5566960.9323836763450752Illumina Small RNA Adapter 2 (100% over 21bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC5441600.9113877256526653No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG5306590.8887755423168143No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA5016640.840213194651975No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA4973060.8329141875430468No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA4476410.7497326391114466RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG3979420.6664941456955178No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG3766770.6308784077030083No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC3697170.6192214344404706No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC3125010.5233930749305049No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA2908830.48718611401246736RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA2807220.4701679379606504RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2653830.4444773757661006Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG2579890.4320935165271344No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA2395400.4011941631190081RNA PCR Primer, Index 1 (100% over 23bp)
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC2275920.38118302568498497No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG2012180.3370104663708799Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC2006810.3361110705889858No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1985740.33258215641309974No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA1834970.3073304055683753No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA1749920.2930857852238518RNA PCR Primer, Index 1 (100% over 23bp)
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1729260.28962553999965596No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA1700260.2847684678069319RNA PCR Primer, Index 1 (100% over 22bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG1667430.27926992711427223Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA1665850.2790053004223928RNA PCR Primer, Index 1 (100% over 23bp)
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1594350.2670301051886076Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1584190.2653284550686739No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1569340.26284129913550314No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1546430.2590042121032511Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG1503200.25176382483113174Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA1444310.24190061857494138No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1426130.23885573676584748Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1424210.23853416508963954RNA PCR Primer, Index 1 (100% over 23bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1418720.23761467107798248No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC1357310.22732940199676918No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1228090.20568695824698283No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG1175190.19682698862646203Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1174320.1966812764606803RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1151190.1928073426738628No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA1134590.19002708755664832No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA1122470.1879971663505857No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG1028240.172215031429193No Hit
TTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTGGAATTCTCGGGTGCC1022490.17125199125304943No Hit
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA1005200.16835617131469774No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG948110.1587944385049523Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC936440.15683988566050092No Hit
CGGACGTTGACTGTCACTGATTTCTGCCCATGGAATTCTCGGGTGCCAAG929890.1557428572859374No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA926680.15520522963977726RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCA910280.15245847157216777No Hit
ATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGGA900940.1508941593556146RNA PCR Primer, Index 1 (100% over 22bp)
CATCCGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG900840.15087741083081208Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCCGGTGCCAAGGA863440.14461346255467827RNA PCR Primer, Index 1 (95% over 22bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG841530.14094386077045123No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAG832020.1393510760617338No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA827860.13865433742994993No Hit
CCACGCGGCCCGGGTTCGATTCCCGGCATGGGATGGAATTCTCGGGTGCC818930.13715869416508697No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACTGGAATTCTCGGGTGCCA818750.13712854682044248No Hit
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA807990.1353264055516938RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG804850.1348005018728954No Hit
GCGTTCGTCGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG798500.13373697054793687No Hit
GCGTTCGTGGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG784370.13137040399334407No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCAA769160.1288229533708843No Hit
CACCCGGTCCGGACACCATTAGGACTGACAGATGGAATTCTCGGGTGCCA750210.12564910792081116No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA743180.12447168662719564No Hit
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA726970.12175675075671091RNA PCR Primer, Index 1 (100% over 22bp)
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG725820.12156414272148221No Hit
AGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCAA719910.12057430490565464No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC714390.11964978633655682RNA PCR Primer, Index 1 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC712980.11941363213684161RNA PCR Primer, Index 1 (100% over 24bp)
CACCGGTCCGGAGTTTGTGGGTTACCAAAATGGAATTCTCGGGTGCCAAG708800.11871354380009724No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC689010.11539901074168313No Hit
ACTCTGGTGGAAGTCCGTATCGGTTCTGACTGGAATTCTCGGGTGCCAAG687610.11516453139444817No Hit
AATCGATCGATAGACTTGGGTATAGGGTGGAATTCTCGGGTGCCAAGGAA687020.11506571509811345RNA PCR Primer, Index 1 (100% over 23bp)
TAGTCGATAGAATAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAGGA675370.11311451195862257RNA PCR Primer, Index 1 (100% over 22bp)
CGTTCGTGGTGTAATGGTCAGCATGGATGCTGGAATTCTCGGGTGCCAAG666210.11158034708671385No Hit
AGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCCA649980.10886206151126862No Hit
ACTTCATCTAAGGCTAAATATCGACTCGATGGAATTCTCGGGTGCCAAGG644030.10786552428552007Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG641040.10736474339392542No Hit
CGTTCGTCGTGTAATGGTCAGCATGGATGCTGGAATTCTCGGGTGCCAAG623520.10443040184852798No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA621980.10417247456656954RNA PCR Primer, Index 1 (100% over 22bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACTGGAATTCTCGGGTGCCAA616960.10333169862148418No Hit
AACTATGCCTGAGCAGGATGAAGCCAGAGGTGGAATTCTCGGGTGCCAAG609220.10203536280177093No Hit
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA601500.10074237668701819RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)