FastQCFastQC Report
Wed 27 Sept 2023
SRR3356506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66043580
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA40749176.170042568861349RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG36694505.556104014954974Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG16119802.4407822834558637No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA9926891.5030817529879514RNA PCR Primer, Index 1 (100% over 23bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA8280751.2538311823798771RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA8029071.2157230119869336No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC6810891.0312720782247118No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA6546610.9912560766693749No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG6306540.9549058364189221Illumina Small RNA Adapter 2 (100% over 21bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC5469980.8282379604497516No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG5211890.7891592188067333No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG4919190.744839998073999No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA4420410.6693171387741246RNA PCR Primer, Index 1 (100% over 23bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG3821960.5787027293190344No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA3646410.5521217959414072RNA PCR Primer, Index 1 (100% over 23bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG3470880.5255438908672122Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG3450070.5223929411458313No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC3189420.4829265766634698No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA3073160.4653230488111032RNA PCR Primer, Index 1 (100% over 22bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA2744890.41561799042389885RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA2670700.4043844988415225RNA PCR Primer, Index 1 (100% over 22bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG2591760.3924317851939583No Hit
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA2584950.3914006478752364RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2518260.3813027700799987Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG2313330.35027325896021994Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG2312940.35021420704328865Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC2310130.34978873041104075No Hit
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA2300100.34827003623970715No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA2159560.32699014802044346RNA PCR Primer, Index 1 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG1993560.3018552295317728Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA1965260.2975701801749693No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1909110.28906821828859064No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC1745330.2642694414809131RNA PCR Primer, Index 1 (100% over 24bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1739000.26331098344456794No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA1728890.26178017605950493RNA PCR Primer, Index 1 (100% over 23bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG1663060.2518125153118592Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1558940.23604716764294123No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA1536070.23258430266802618No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1503430.2276421114663984No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1459260.22095410333600934No Hit
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA1455460.22037872568385902RNA PCR Primer, Index 1 (100% over 23bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA1437590.21767293656703648No Hit
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1430220.21655700675220815RNA PCR Primer, Index 1 (100% over 23bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1391330.21066847072796477Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG1320430.1999331350602133Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCA1311850.19863399288772657No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG1309680.19830542196531442Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1293270.19582069899905488No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1250520.1893477004123641Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC1217740.18438431108670972No Hit
TTCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG1201810.18197226740282704No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA1058970.1603441242888408No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1054100.15960673240305873No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA1029030.15581075405058295No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1010690.15303379980309972Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1008880.15275973834247022No Hit
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA966560.1463518482795754RNA PCR Primer, Index 1 (100% over 22bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG952700.14425323400094303No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC942120.1426512614852193RNA PCR Primer, Index 1 (100% over 24bp)
TCCTCGGTAGTATAGTGGTGAGTATCCGCTGGAATTCTCGGGTGCCAAGG926870.1403421801180372Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAA923090.1397698307693193No Hit
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA916040.13870235380940887No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGGA904060.1368884000534193RNA PCR Primer, Index 1 (100% over 22bp)
ATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG898390.1360298760303424RNA PCR Primer, Index 12 (100% over 37bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG883140.13372079466316028Illumina Small RNA Adapter 2 (100% over 21bp)
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA861440.1304350854390389RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA860760.13033212312233833RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG853050.1291647121491597No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA833050.12613640871678974No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACTGGAATTCTCGGGTGCCA832580.12606524358612903No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA819780.12412712938941226No Hit
GCGTTCGTGGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG816910.12369256784686716No Hit
AAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA816490.12362897347478741RNA PCR Primer, Index 12 (100% over 40bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA806420.12210422269658913RNA PCR Primer, Index 1 (100% over 22bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA781970.11840212175051686No Hit
CTCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG778010.1178025176709076No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG769170.11646400755380007No Hit
ATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGC765280.11587500253620413No Hit
TCTGTTGAGACTATCCTTTGATTGAGTTTGGAATTCTCGGGTGCCAAGGA753870.11414735542803706RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGA750380.1136189164790885RNA PCR Primer, Index 1 (100% over 22bp)
CGGACGTTGACTGTCACTGATTTCTGCCCATGGAATTCTCGGGTGCCAAG734890.11127349547071796No Hit
ATCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGA720450.10908706039254686RNA PCR Primer, Index 1 (100% over 22bp)
AATCGATCGATAGACTTGGGTATAGGGTGGAATTCTCGGGTGCCAAGGAA717460.10863432902940755RNA PCR Primer, Index 1 (100% over 23bp)
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC710470.10757593697979426No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA699890.10597396446407055No Hit
AGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCAA696980.10553334631466071No Hit
TGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAAC692220.10481261009775668RNA PCR Primer, Index 1 (100% over 24bp)
AGGAAGGTCGTGGCGGTTGCGTTGAAGGCTTGGAATTCTCGGGTGCCAAG683160.10344078864289306No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC670140.10146936310842024No Hit
CACCCGGTCCGGACACCATTAGGACTGACAGATGGAATTCTCGGGTGCCA668300.1011907591926422No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)