FastQCFastQC Report
Wed 27 Sept 2023
SRR3356507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65527707
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG34614655.282444874806927Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA29319504.474366850651435RNA PCR Primer, Index 1 (100% over 22bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG22859453.4885166972193917No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA10992261.6774980391119136No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC8381421.2790650525891285No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC7445171.1361865599844658No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA6153320.9390409464503313RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA5997690.915290687647593RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG5679710.866764649646599No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA5663710.8643229344191763No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG5042970.7695935400272743Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG4973060.7589247705554537No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC4848440.7399068610778644No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA3377230.5153896198443202No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG3358460.5125251826681498No Hit
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG2657090.405491069602054Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG2642250.40322637872861933Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2614600.3990067896012293No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA2588150.39497032911589597No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG2574030.3928155154276953No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG2565640.39153514100531545No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2520730.3846815515763431RNA PCR Primer, Index 1 (100% over 22bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC2387360.3643283290837569No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG2326910.35510322374015013Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2276080.3473461996770313Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA2266970.34595594806941743RNA PCR Primer, Index 1 (100% over 23bp)
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA2200620.33583046023569846RNA PCR Primer, Index 1 (100% over 22bp)
ACAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCA2104420.32114964743081886No Hit
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA2057010.31391453999756164No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAA2011900.30703042912824646No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG2010680.30684424834215546Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA2006030.30613462485418574RNA PCR Primer, Index 1 (100% over 23bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG1972230.30097650143625504Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1882730.2873181568828587RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1875620.2862331196786727No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC1785700.27251068010055657No Hit
TTCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG1785560.2724893150923166No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1728210.263737292074023RNA PCR Primer, Index 1 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA1679610.2563205820707262RNA PCR Primer, Index 1 (100% over 23bp)
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA1644530.2509671214346017No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1637650.2499171838868099No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC1583970.24172522929880638No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCGCTGGAATTCTCGGGTGCCAAGG1558900.2378993667518383Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA1470120.22435089938367597RNA PCR Primer, Index 1 (100% over 23bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1442290.22010384095997745No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA1436510.21922177133407098No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1418320.21644584633489466No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1382990.21105423389834163No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1309380.19982081778017963No Hit
TTCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA1202490.18350863398897813No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC1146420.17495194818887833No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG1132890.17288717274968893No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCA1084730.16553760991514627No Hit
ATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGC1070320.16333854013844862No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG1040350.15876490230308227Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC994390.15175107531231027RNA PCR Primer, Index 1 (100% over 24bp)
GCGTTCGTGGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG975780.1489110552884141No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG966900.14755590333719446Illumina Small RNA Adapter 2 (100% over 21bp)
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA963570.14704772135548708RNA PCR Primer, Index 1 (100% over 23bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACTGGAATTCTCGGGTGCCA936940.14298379157384525No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA928350.14167289571112263No Hit
CTCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG912190.1392067633314256No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG908410.13862990810894696Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG907340.13846661840311306Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC872150.13309637097480004No Hit
TAGTTGGCGAATCTTCGGGTTCGTATAACTTCTGGAATTCTCGGGTGCCA870650.13286746017222914No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG868800.13258513684905837Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG809540.12354163407549115No Hit
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA807740.12326694111240609RNA PCR Primer, Index 1 (100% over 22bp)
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA805490.12292357490854976No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAA801620.12233298503791686No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCCGGTGCCAAGG776840.1185513785794458Illumina Small RNA Adapter 2 (95% over 21bp)
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC770810.11763115715311082No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA770290.11755180140821958No Hit
CACCCGGTCCGGACACCATTAGGACTGACAGATGGAATTCTCGGGTGCCA750140.11447676629368399No Hit
GCGTTCGTCGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG742780.1133535772890695No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGGA738530.11270499668178532RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCC737860.11260274985663697No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA725600.11073178556362424No Hit
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA724280.11053034405736188RNA PCR Primer, Index 1 (100% over 22bp)
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG721620.11012440890080283Illumina Small RNA Adapter 2 (100% over 21bp)
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA704070.10744615251072345No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG694950.10605437483109245No Hit
AATCGATCGATAGACTTGGGTATAGGGTGGAATTCTCGGGTGCCAAGGAA687260.10488082544991237RNA PCR Primer, Index 1 (100% over 23bp)
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCAA672750.10266649495304331No Hit
AGGAAGGTCGTGGCGGTTGCGTTGAAGGCTTGGAATTCTCGGGTGCCAAG660250.10075890493161924No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCA656940.10025377509394615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)