FastQCFastQC Report
Wed 27 Sept 2023
SRR3356509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67919114
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA50724957.468435174227979RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG45623136.717274020977364Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG19431752.8610134696397833No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA10104051.487659276591859No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG8019841.1807927883158194Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG6176320.9093640414685032No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC6031850.888093151509603No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC5566090.8195174630811587No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA5531510.8144261128023548RNA PCR Primer, Index 1 (100% over 22bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC5512420.8116154165379719No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA4892410.7203288900382299RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA4722380.6952947001045979No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA4551550.6701427230043078RNA PCR Primer, Index 1 (100% over 23bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG4235970.6236786304367868Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA3361540.49493284026054873RNA PCR Primer, Index 1 (100% over 23bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC3340270.491801173967022No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG3033190.4465885700452453No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2951010.4344888833502746RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG2791250.41096678616861815No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG2732730.4023506549275658No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG2717740.40014361789230646No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2436490.35873406711400857No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2410050.3548412012559528Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG2332440.3434143737505174Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA1976230.29096816545633974RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1882670.2771929563156551RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA1866250.27477537471999414No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA1862410.27420999632003445No Hit
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA1791070.2637063257332833No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1776440.2615522929230202RNA PCR Primer, Index 1 (100% over 22bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA1776170.26151253975427297RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1770850.2607292550959955No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA1662780.24481768122004655No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1657630.24405942633468394No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG1631920.24027404126620378Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG1583550.23315233470212818Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG1526250.22471582889022962Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1524820.22450528432982797No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA1520910.22392959955278569No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1460630.2150543365450851No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1448030.2131991886702173Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1363830.20080208937943447No Hit
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1294700.19062380584057678RNA PCR Primer, Index 1 (100% over 23bp)
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1248020.18375092466606677No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC1233140.18156008336622295No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA1195440.176009363137452RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG1188960.17505528708752002No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC1159920.17077961293782482No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG1103380.16245500493425163No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA1090450.16055126985313736No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA1087850.160168461561498No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1049250.1544852307702365No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1047040.154159843722343Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC1029440.15156852605586107No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG1026830.15118424542463849Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA1022570.15055702876218321No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAA1001340.14743125182698938No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA953050.1403213239795796RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG931240.13711015134855853No Hit
GCGTTCGTGGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG914370.13462631447165227No Hit
CTTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA911690.13423172746334708RNA PCR Primer, Index 1 (100% over 22bp)
ACAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCA907620.1336324852529731No Hit
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA879320.12946576423243683No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCGCTGGAATTCTCGGGTGCCAAGG847080.12471894141610858Illumina Small RNA Adapter 2 (100% over 21bp)
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA844270.12430521399322142RNA PCR Primer, Index 1 (100% over 22bp)
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG837290.123277520964128Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG826720.12172125802465561Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC789990.11631335473545783No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG785040.11558454664175978Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC769700.11332597772108746No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG751480.1106433750004454Illumina Small RNA Adapter 2 (100% over 21bp)
AACTATGCCTGAGCAGGATGAAGCCAGAGGTGGAATTCTCGGGTGCCAAG735130.108236099781867No Hit
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA731590.10771489156940417RNA PCR Primer, Index 1 (100% over 22bp)
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG726900.10702436430487004No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTCCCAAGG725750.10685504525279879Illumina Small RNA Adapter 2 (95% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCCGGTGCCAAGG718700.10581704584662278Illumina Small RNA Adapter 2 (95% over 21bp)
GCGTTCGTCGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG699700.10301960063848889No Hit
TAGTTGGCGAATCTTCGGGTTCGTATAACTTCTGGAATTCTCGGGTGCCA691340.10178872474690998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)