FastQCFastQC Report
Wed 27 Sept 2023
SRR3356510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61554530
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA60320679.799550089977132RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG26442434.295773194921641Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG8579781.3938502982639944No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG7304431.1866600232346831Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA7096201.1528314812898417RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA6307711.0247353038029858No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA5464290.8877153314305217RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA5324080.8649371540973507RNA PCR Primer, Index 1 (100% over 23bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG4647910.7550882120292366Illumina Small RNA Adapter 2 (100% over 21bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC4642640.7542320605810815No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA4574850.7432190612128791No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG4530830.7360676785282902No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG4389990.7131871529195333No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC4178030.6787526442001913No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC3798490.6170934941750023No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG3651610.593231724781263No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA3234160.525413807887088RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA3084110.5010370479638135RNA PCR Primer, Index 1 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA2982230.4844858696833523RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG2782080.451969984987295No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC2522630.40982036577974035No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG2401910.3902084866865201Illumina Small RNA Adapter 2 (100% over 21bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG2219850.36063145961800047Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA2218490.3604105173087992RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2193960.35642543286416123RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC2111690.34306004773328624No Hit
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1984150.3223402079424536RNA PCR Primer, Index 1 (100% over 23bp)
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA1962060.31875151999373563RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1870760.3039191429127962Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAAC1870310.3038460370016634RNA PCR Primer, Index 1 (100% over 24bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1677050.2724494850338391No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1560940.2535865353857791No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1363930.2215807674918483Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1353060.21981485359404093No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1340370.21775326690009655No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1272120.20666553704495835RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1241110.20162772748000837No Hit
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA1221850.1984987944835254No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA1216970.19770600149168552No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1160220.18848653380994057Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA1106530.1797641863238985No Hit
CTTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA1063910.1728402442517228RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA1063070.17270377988427496No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG1026520.16676595532449034No Hit
GTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAAC1021990.1660300224857537RNA PCR Primer, Index 1 (100% over 24bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA1018790.1655101582288095RNA PCR Primer, Index 1 (100% over 22bp)
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA1014850.1648700753624469RNA PCR Primer, Index 1 (100% over 22bp)
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG999390.1623584811710852No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC982420.15960157603347797No Hit
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAG969240.157460385125189No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG951440.15456864019593686Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG937270.15226661628315577Illumina Small RNA Adapter 2 (100% over 21bp)
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA932300.15145920210908928No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGTGGAATTCTCGGGTGCCA928750.1508824776990418No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA919130.1493196357766033No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC908300.14756022018200773No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG867060.14086046957063925Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGGA858880.1395315665638256RNA PCR Primer, Index 1 (100% over 22bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG849010.1379281102463133Illumina Small RNA Adapter 2 (100% over 21bp)
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA822770.1336652233393708No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAAC790310.128391850282993RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC778510.12647485083551122No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG754770.12261810787930637No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTGGAATTCTCGGGTGCCAAGGA736910.11971661549523649RNA PCR Primer, Index 1 (100% over 22bp)
TCTGTTGAGACTATCCTTTGATTGAGTTGGAATTCTCGGGTGCCAAGGAA722240.1173333627923079RNA PCR Primer, Index 1 (100% over 23bp)
CCACGCGGCCCGGGTTCGATTCCCGGCATGGGATGGAATTCTCGGGTGCC719360.1168654849610581No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG701820.11401597900268266Illumina Small RNA Adapter 2 (100% over 21bp)
CATCCGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG695140.11293076236631164Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC694010.1127471853005782No Hit
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG657170.106762248042508No Hit
TTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGGAACT651790.10588822626052055RNA PCR Primer, Index 1 (100% over 25bp)
CGGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA620750.10084554296816173RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)