FastQCFastQC Report
Tue 26 Sept 2023
SRR3356512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66159200
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA45212576.833905186277947RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG26238343.9659397332494954Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA19015522.874206459570249No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC14186352.1442747191622633No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG7186821.0862918535895236Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG7019121.0609439050048972No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA6979941.0550218261405822No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA6769991.023287766478434RNA PCR Primer, Index 1 (100% over 22bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC6317560.9549027195008404No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC5126240.7748340366872634No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA4784130.72312391927351RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA4672480.7062479594674663RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG4444940.6718551614892562No Hit
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC4361530.6592476934424842No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA3912230.5913357477115805RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG3513880.5311249229132153No Hit
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG3442980.5204083483476221Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG3286600.4967714240800977No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC3151180.47630261550925646No Hit
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1995310.30159221997847613RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA1849760.2795922562546101RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA1849530.2795574916262591RNA PCR Primer, Index 1 (100% over 22bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA1840570.2782031826261503RNA PCR Primer, Index 1 (100% over 23bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1803810.2726468881123109No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1712430.2588347501178974Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG1627010.2459234694494492Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCCGGTGCCA1594710.2410413064245033No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCGGGTGCCA1560900.23593090605690514No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1524300.23039879563235346No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA1497860.22640237487756804No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1495740.22608193569450658Illumina Small RNA Adapter 2 (100% over 21bp)
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA1495740.22608193569450658No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1451410.21938143145624495No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG1260250.19048749077981594Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA1253570.18947780505205625No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC1248820.1887598399013289No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1242580.18781666041911027No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA1209790.18286043362072094No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1205340.18218781363740794No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCCAA1153670.174377864303075No Hit
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1117030.1688397078562014Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA1093280.16524988210256472RNA PCR Primer, Index 1 (100% over 23bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG1083220.16372930748860323Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG1069830.1617054015163424Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1066730.16123683478639403Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA1055380.1595212759525508RNA PCR Primer, Index 1 (100% over 22bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1005370.15196223654457733No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG997100.1507122214295215No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG980220.14816080000967363No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA957440.14471759029734338No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG930540.14065164028585594Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA924180.13969032273667153RNA PCR Primer, Index 1 (100% over 22bp)
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC922940.1395028960446922No Hit
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC893680.1350802307162118No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAG865020.13074825572256013No Hit
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC864340.13064547334308757No Hit
ATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGC861330.13019051016336353No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG836950.1265054595581567No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC833510.12598550163847205No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA812930.1228748231538471RNA PCR Primer, Index 1 (100% over 23bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA791470.11963113217813999No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCC784640.11859877386667311No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCCGGTGCCAAGGA750120.11338105660286096RNA PCR Primer, Index 1 (95% over 22bp)
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG737480.11147051354913602No Hit
CGGACGTTGACTGTCACTGATTTCTGCCCATGGAATTCTCGGGTGCCAAG737100.11141307633707784No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG718860.10865609015828487No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGTGGAATTCTCGGGTGCC708490.10708865887132854No Hit
TTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTGGAATTCTCGGGTGCC697350.10540484165467538No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACTGGAATTCTCGGGTGCCA682760.1031995550127571No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA675300.10207197184972008No Hit
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA675190.1020553452883348No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)