FastQCFastQC Report
Wed 27 Sept 2023
SRR3356515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62944837
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA41421226.580558783558373RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC40276416.398683660107024No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG33557925.331322090801506Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA22488413.572717171386114No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG11399271.8109936482955704No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA7856761.2481976877626992No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG7805981.2401303064777178Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG4848270.7702410922122175No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA4259700.676735408815182RNA PCR Primer, Index 1 (100% over 22bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG4255690.6760983430618781No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC3789850.6020906845783078No Hit
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC3703000.5882928888988942No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA2853600.4533493350693719RNA PCR Primer, Index 1 (100% over 23bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC2636550.4188667610657249No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG2595790.4123912498176776No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC2519030.4001964450237595No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA2385030.3789079634919064No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2300270.3654422045766836RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA2221540.3529344273303941RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA1937290.3077758387077879RNA PCR Primer, Index 1 (100% over 23bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG1778090.28248385169382517Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG1596760.25367608784180345No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1542550.24506378497731277No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA1536390.24408515030390818No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA1437620.22839363298375054RNA PCR Primer, Index 1 (100% over 23bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCGGGTGCCA1436090.228150563008051No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGGAATTCTCGGGTGC1396220.22181644540599893No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1385990.22019121282338058No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCA1344810.21364897648396483No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG1295340.20578971393634715Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG1233220.19592075518441646Illumina Small RNA Adapter 2 (100% over 21bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG1186830.18855080997350107Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG1185160.18828549830067873Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1181140.18764684385472313No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG1177820.1871193978943817Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA1169880.18585797592898684RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA1167580.18549257661911173RNA PCR Primer, Index 1 (100% over 22bp)
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA1151770.18298085353688343No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1129580.17945554454291462No Hit
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA1125100.178743810234984No Hit
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGTGGAATTCTCGGG1122460.17832439537495348No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG1119680.1778827388178001No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGGA1042030.16554654037788677RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCA1038280.1649507806335252No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1027460.16323181518446064No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG1011040.16062318185048283Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA938260.14906067673191367No Hit
TCTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGC918590.14593571828615587No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA915140.14538761932134323No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG908670.14435973517573808Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG908440.14432319524475057Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAA890100.14140953292165964No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG887160.14094245728208019Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA850300.13508653616816899RNA PCR Primer, Index 1 (100% over 22bp)
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC824060.13091780665028968No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG819680.1302219592688754Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA814990.12947686241526052RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG810010.12868569347474837Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCCGGTGCC802240.12745127928443123No Hit
TGAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAG792810.12595314211394337No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAG761520.1209821228069905No Hit
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA740960.11771577071523753No Hit
ATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGC737560.11717561521368307No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCCGGTGCC714460.11350573518841586No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA696690.11068262834646789RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG650690.10337464214896608No Hit
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTTGGAATTCTCGGGTGC649280.10315063648508616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)