FastQCFastQC Report
Tue 26 Sept 2023
SRR3356518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64330398
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGG37625715.848822822454791Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA32498555.051818581940065RNA PCR Primer, Index 1 (100% over 22bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAG23086633.5887590808936083No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTTGGAATTCTCGGGTGCCA10785081.6765137998990771No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTTGGAATTCTCGGGTGCCAA7785701.210267656046524No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCC7651781.1894501258953816No Hit
TGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA6235800.9693395647886401RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAG5858220.9106456950569464No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAAGG5747050.8933645956923817Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGAA5571380.8660571321197174RNA PCR Primer, Index 1 (100% over 23bp)
CCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCC5018140.7800573532904305No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGGAATTCTCGGGTGCC4411010.6856805083033995No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAG4216640.6554661763479219No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG3923600.6099138388666584No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTGGAATTCTCGGGTGCCAAG3065910.47658806650007046No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGA2787450.43330215367235875RNA PCR Primer, Index 1 (100% over 22bp)
CATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAGG2721010.42297422130048073Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGCC2718470.42257938463244077No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA2705350.4205399133392584No Hit
CTGTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGG2601740.4044339971283871Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTTTGGAATTCTCGGGTGCCAA2564670.3986715580401041No Hit
TTTGGTGTCTCGTGCTCTTTGAACGGCCTGGAATTCTCGGGTGCCAAGGA2477320.3850932182947166RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATGGAATTCTCGGGTGCCAAGGAA2392800.37195479499442863RNA PCR Primer, Index 1 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGG2310680.35918944571118616Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCTGGAATTCTCGGGTGCCAAGG2221020.3452520222243923Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGGA2200840.3421150915310675RNA PCR Primer, Index 1 (100% over 22bp)
CAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCAA2127530.3307192347853965No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA2115000.3287714775214044RNA PCR Primer, Index 1 (100% over 23bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGG2105670.3273211522801398Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGAAGGTCGGTGGTTCGAGCCCGCCCGAGATGGAATTCTCGGGTGCCA2074590.3224898437593997No Hit
CGCGGGCGGCCCGGGTTCGTTTCCCGGTCGATGCTGGAATTCTCGGGTGC1839710.28597833329120703No Hit
CCCATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGC1733250.26942939168509417No Hit
CCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGCCA1728730.26872676895299175No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTGGAATTCTCGGGTGCCAAGGA1694620.2634244544857316RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCATGGAATTCTCGGGTGCCAAG1587750.2468117794017068No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAA1576950.24513294632500177No Hit
TTCGGACGCTTTCTGGTGTGTGCTTGGACCTGGAATTCTCGGGTGCCAAG1527150.23739166047130628No Hit
CGCCTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAG1517610.23590869125355013No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTGGAATTCTCGGGTGCCAAGGAA1501340.2333795603129954RNA PCR Primer, Index 1 (100% over 23bp)
CTGGTTGTCGCGGGTTCGAGCCCCGCATTGGGCTGGAATTCTCGGGTGCC1421210.2209235515688866No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAA1419200.22061110207961093No Hit
CCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTTGAATTCTCGGGTGCCA1385630.21539272926618613No Hit
AAGCGAGAGACCTATACTCCGCCGTTGCGACATGGAATTCTCGGGTGCCA1317440.20479276375687896No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGCTGGAATTCTCGGGTGC1296310.20150815793180699No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTGGAATTCTCGGGTG1280160.19899768069210452No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCGCTGGAATTCTCGGGTGCCAAGG1181080.18359594168840676Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTGTCTCGTGCTCTTTGAACGGCCCTGGAATTCTCGGGTGCCAAGG1123600.17466081898016547Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATGGAATTCTCGGGTGCCAAGGAA1108170.1722622639455767RNA PCR Primer, Index 1 (100% over 23bp)
CGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCCAA1100500.17106998156610193No Hit
ATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGTGGAATTCTCGGGTGC1055910.16413857722440953No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTGGAATTCTCGGGTGCCAA1046780.16271934148456535No Hit
TTCGGACGCTTTCTGGTGTGTGCTTGGACCTTGGAATTCTCGGGTGCCAA1043330.16218304758506236No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAAGG1027010.15964614426915252Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTGGAATTCTCGGGTGCCAAGG1021970.15886268883335683Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTTTTTTCGAAGCATTAAGCGGAGCCTGGAATTCTCGGGTGCCAAGG998060.15514593893854037Illumina Small RNA Adapter 2 (100% over 21bp)
GCATCGGTGGTTCAGTGGTAGAATGCTCGCTGGAATTCTCGGGTGCCAAG996360.15488167817646645No Hit
CTTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGA984170.15298677306488917RNA PCR Primer, Index 1 (100% over 22bp)
GAACTGATTCGAGAAGAGTGGGGACTGTCTGGAATTCTCGGGTGCCAAGG978210.15206030592255934Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTGTCTCGTGCTCTTTGAACGGCCCTTGGAATTCTCGGGTGCCAAGG926260.1439848079285939Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCCA917120.1425640177136787No Hit
GCGTTCGTGGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG913320.14197331718668987No Hit
CCAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC911610.14170750194954493No Hit
TTTGGTCGTGACTTCTAATTTGCTGGTTGGAATTCTCGGGTGCCAAGGAA902930.1403582175878968RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAA880700.13690261950501223No Hit
ACAGAAGGTCGCTGGTTCGATTCCAGCATGAGGTGGAATTCTCGGGTGCC869680.13518958797674466No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACTGGAATTCTCGGGTGCCA848930.13196405220437157No Hit
GACGGCCTGGGAATCCTGGATGTTGTAAGCTTGGAATTCTCGGGTGCCAA845320.1314028867037322No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTTGGAATTCTCGGGTGCCAAGG796250.1237750775302214Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTTTGGAATTCTCGGGTGCCAAG787150.1223605052155903No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGGCTTGGAATTCTCGGGTGCC757830.1178027843073503No Hit
CATAAGGCCGGGGTTCGATTCCCCGCAACGGATGGAATTCTCGGGTGCCA746190.11599337532467932No Hit
CACCCGGTCCGGACACCATTAGGACTGACAGATGGAATTCTCGGGTGCCA743840.11562807368298887No Hit
TAGTTGGCGAATCTTCGGGTTCGTATAACTTCTGGAATTCTCGGGTGCCA725380.11275851270188006No Hit
TCCGTTGTGGTCTAGTGGTTAGGATTTATGTGGAATTCTCGGGTGCCAAG723270.11243051846189418No Hit
GCGTTCGTCGTGTAATGGTCAGCATGGATGTGGAATTCTCGGGTGCCAAG710230.11040348296927993No Hit
AGGAAGGTCGTGGCGGTTGCGTTGAAGGCTTGGAATTCTCGGGTGCCAAG704620.10953142245443592No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATTTGGAATTCTCGGGTGCCAAGGA694780.10800181898454912RNA PCR Primer, Index 1 (100% over 22bp)
TCTATTAATTAATCTGACGTGATATTAGTGGAATTCTCGGGTGCCAAGGA687390.10685306190706297RNA PCR Primer, Index 1 (100% over 22bp)
TTGTGATCGTTGCCGGGTGTCGTTTCCTTGGAATTCTCGGGTGCCAAGGA676690.10518977358106817RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)