FastQCFastQC Report
Tue 4 Jul 2023
SRR3356523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44716778
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length20-39
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGC6666451.4908162658767588No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGC6364751.4233471830193132No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT6279691.4043252400698458No Hit
ATGTACACGGAGTCGACCCGCAACG5075561.1350459999600149No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC4158540.9299730852701418No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGG4036920.9027752402017873No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC3671180.8209849108538188No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGC3506150.7840793001678251No Hit
TCGACTGCATAATTTGTGGTAGTGGGG3106160.694629653326096No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCC2976970.6657389313693397No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2894740.6473498604930793No Hit
ACGGTGGCCATGGAAGTCGGAATCCGC2415040.5400746896388644No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCG2344560.5243132678298066No Hit
CCGCCTGGATACCGCAGCTAGGAATAATG2298130.5139301404944695No Hit
AAATCAGTTATGGTTCCTTTGGTCGC2172910.4859272284778658No Hit
CCGCCACGCAGTTTTATCCGGTAAAG1891010.4228860138357911No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC1813780.40561509149876585No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGG1757050.39292857817260446No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCG1518440.33956829358322727No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTT1456420.32569877910255524No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGG1391500.31118073846912675No Hit
TCGACTGCATAATTTGTGGTAGTGGG1389560.3107468968359035No Hit
CCGCCGAGGGCGCACCACCGGCCCGTGT1277640.2857182599336652No Hit
CTAAACCATTCGTAGACGACCTGCT1265710.2830503575190502No Hit
ATTAAATCAGTTATGGTTCCTTTGGTGGC1194870.26720842901516745No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAG1187800.265627366980689No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC1148520.2568431920564581No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC1085880.24283502715692082No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGG1078440.24117122213053901No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTAT1058340.23667626500281394No Hit
TATCCGGTAAAGCGAATGATTAGAGG1038050.2321388182305979No Hit
CTAAACCATTCGTAGACGACCTGCTT965680.21595473627370915No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGAC946100.21157606659406455No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGAT931990.20842065141634308No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCG870580.1946875510574577No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGC869920.19453995545027863No Hit
CCGCCGAGGGCGCACCACCGGCCCGTGTC855630.19134428692514474No Hit
TTTTATCCGGTAAAGCGAATGATTAGAGG803310.1796439806105887No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGGGG771700.17257504554554445No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC736500.16470327982932936No Hit
TTTATCCGGTAAAGCGAATGATTAGAGG719430.16088592071638078No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGG716660.16026646642564454No Hit
CCGCCTGGATACCGCAGCTAGGAATAAT710650.15892245188148393No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGC707970.15832312426445394No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGGGGC701900.15696569193782253No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCT690150.15433804287062006No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAC654800.14643273269822793No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCG649550.14525867673203108No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGC625770.139940762279429No Hit
TAAATCAGTTATGGTTCCTTTGGTCGC616230.13780733486656843No Hit
AACTTCTTAGAGGGACAAGTGGCGTT607220.1357924311988668No Hit
TCTGGCATGCTAACTAGTTACGCGACCC598170.13376858234285124No Hit
TTATCCGGTAAAGCGAATGATTAGAGG596000.13328330587682324No Hit
CCCGCCACGCAGTTTTATCCGGTAAAG593430.1327085775276564No Hit
CCGCCGAGGGCGCACCACCGGCCCGTGTCG591500.13227697219151166No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAG587190.131313128150691No Hit
AACTGTGGTAATTCTAGAGCTAATACATG585310.1308927042999386No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGT575250.1286429894389976No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCT559570.12513647561995633No Hit
CCTCGCCCGTCACGCACCGCACGTTC550350.12307460971360683No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT545240.12193186190650855No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC539900.12073767926660547No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGC521360.11659158448312176No Hit
ACTGTGGTAATTCTAGAGCTAATACATG498590.11149953603544513No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCC478660.10704259595805406No Hit
AAAAAGCTCGTAGTTGGATCTTGG468620.10479735369126998No Hit
TTAAATCAGTTATGGTTCCTTTGGTCGC466290.10427629647198641No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGT453880.10150105179760492No Hit
CGGTCGAACTTGACTATCTAGAGGAAG452330.10115442575044205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)