FastQCFastQC Report
Sun 2 Jul 2023
SRR3356524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60694250
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length20-40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACG15161632.4980339982782556No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG8513621.4027061871594095No Hit
ATGTACACGGAGTCGACCCGCAACGC5716660.9418783492670227No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCG2516220.41457304439876924No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1992500.32828480457374465No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC1560490.25710672757304026No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1549590.25531084081276234No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATG1218750.2008015586319956No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC1217020.20051652339389647No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT1216940.20050334257363753No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC1109040.1827257112494182No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCG1053540.17358151719479192No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC977920.16112234684504712No Hit
CCGGGCCGCCCCTCCCACGGCGCGACCGC899810.14825292346474336No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC889470.14654930244627787No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCG883280.14552943647874386No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGC741550.12217796578753341No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCG723680.11923370006219701No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC672970.11087870762057361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)