FastQCFastQC Report
Sun 2 Jul 2023
SRR3356527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38333571
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length25-35
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC19275955.028477519091556No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG15418834.022278540133921No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT14101323.678582410180361No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC12981403.3864311780397394No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT11619273.031095120253733No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC9774382.549822451970363No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT7888502.057856806505191No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC7259421.8937499978804477No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC6811321.776855070455085No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6219901.62257254874585No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC5663441.4774099704929655No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCC2538340.6621715467103235No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG2322460.605855374131463No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2315000.6039092992406057No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT2019490.5268202119755553No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1788180.46647884696158365No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT1693470.44177204362202516No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTC1604230.41849218795713033No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC1574310.41068701895787374No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC1465500.3823019775538261No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCG1452300.3788585206423894No Hit
ATGTACACGGAGTCGACCCGCAACGCG1430180.37308812163625454No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC1292050.337054432001652No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT1242250.32406320819941353No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1172190.30578679977401535No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCG1114420.290716458427523No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTC1113400.29045037312073013No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1047160.2731704802560659No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGG1034640.26990441354915773No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1020380.2661844366130148No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGC989300.25807666079426833No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGG987650.2576462286803387No Hit
CCTCGCCCGTCACGCACCGCACGTTCG903570.23571245162627816No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTTG900130.23481506588572196No Hit
AGATTAAGCCATGCATGTCTAAGTACGC870770.22715598293725361No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGG870320.22703859236072738No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCT867360.22626642323513246No Hit
AGAAACCTCCCGTGGAGCAGAAGGGC860350.22443773892080132No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT857600.22372035206425198No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCT845430.22054558913908645No Hit
AAGATTAAGCCATGCATGTCTAAGTACGC841420.21949950866826368No Hit
CTCGCCCGTCACGCACCGCACGTTCG840250.21919429316929537No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCC817500.21325954735602382No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGC791640.20651350222498183No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCG782280.20407177823323583No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCG781680.20391525746453415No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGC780330.20356308573495538No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGT768680.20052397414266465No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC740700.19322488896221018No Hit
ATGTACACGGAGTCGACCCGCAACGC690730.18018931760883952No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCG683530.17831106838441949No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCT676630.1765110795443503No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGG670900.17501630620324937No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGC660440.1722876274688836No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCC658660.17182328252173534No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGG640690.16713548549912033No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTC632150.16490767322459993No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCT606170.15813032393981766No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT602460.15716250385334568No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGC597180.155785121088771No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCG596710.15566251315328802No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGC593260.15476251873325342No Hit
AAAAGATTAAGCCATGCATGTCTAAGTACGC586690.15304861631597014No Hit
GAGGGCGCACCACCGGCCCGTCTCGC559270.14589561718630387No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTC546230.14249389914652094No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGC540760.1410669514718574No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGC539820.14082173560089145No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGC531150.13856001049315234No Hit
ACAACTTCTTAGAGGGACAAGTGGCGTTC524010.13669741334560248No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCT510160.13308439226807228No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT505620.13190005178489633No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC482780.12594182785631947No Hit
AAGGGACAAGCATCAAGCACGCAGC474380.1237505370944961No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT469240.12240967584261847No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCC458360.11957143257016155No Hit
CTGGCATGCTAACTAGTTACGCGACCCC437700.11418190076786741No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGC437190.11404885811447099No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG432900.11292973461825406No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCC425590.11102278991957205No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC420100.10959062488595178No Hit
AAAAAGCTCGTAGTTGGATCTTGGG415250.10832541533894663No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCC412610.10763672395665928No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGG408630.10659846952427157No Hit
AAAAGGGACAAGCATCAAGCACGCAGC402090.10489239314542337No Hit
TCGCCCGTCACGCACCGCACGTTCG400060.10436283121131606No Hit
AACTTCTTAGAGGGACAAGTGGCGTTC400010.10434978781392425No Hit
TAGTGACGAAAAATAACAATACAGG397790.10377066096972808No Hit
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGC383640.10007937950784705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)