FastQCFastQC Report
Sun 2 Jul 2023
SRR3356528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38629161
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length25-35
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT31729758.21393713417695No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT25813576.682405035926098No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT20044095.188849429062153No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG13229333.424700319015471No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC11703433.0296878567981325No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT9683492.5067823761432457No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT9662542.5013590121721774No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT8795452.276893872999209No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT7067461.8295660110246765No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT4664361.2074712158516723No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC3984791.0315497144760664No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3547280.9182907182478025No Hit
CGAGGGCGCACCACCGGCCCGTCTCG3535650.915280039346441No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3472760.8989995925616919No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG3176960.8224253174952466No Hit
AAAAAGCTCGTAGTTGGATCTTGGG3025050.7831001040897574No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC2999500.7764859298911514No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC2879890.7455222752572855No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC2707990.7010222147977794No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG2400540.6214320833942005No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC2342330.6063631565800769No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGG2223990.5757282691177269No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCG2109480.5460848606056963No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCC2043190.5289242497397239No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGC2023420.5238063544791978No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGC1897340.49116779937312127No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCC1834960.4750193772005558No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCC1650100.42716433836085643No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1598720.41386350586283766No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGG1505110.38963051773244567No Hit
ACGGGGCTACGCCTGTCTGAGCGTCGCT1471040.38081075589500896No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1312480.339764045095362No Hit
TCGCCCGTCACGCACCGCACGTTCG1294510.33511211905430716No Hit
ATGGGCGAGCGCCGTTCCGAAGGGACGGGCG1176480.3045574818464217No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCG1158130.2998071845257007No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1047730.27122773906479614No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG1015430.2628661802931728No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC988640.25593100507670874No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGGG961700.24895699909195543No Hit
AGGGGCCCGTGCCTTGGAAAGCGTCGCG947890.24538197969145642No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCG919790.2381076824319327No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGC862190.22319666740885208No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCG859400.2224744151186716No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC856420.22170297718865808No Hit
GAGGGGCCCGTGCCTTGGAAAGCGTCGCG854000.2210765074602578No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT853880.2210454428456264No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT802710.20779897342321257No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGC800300.20717509241269827No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGG777610.20130129152947435No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC775750.20081979000268735No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGC754730.1953783050064173No Hit
TGAGGGGCCCGTGCCTTGGAAAGCGTCGCG738290.19112245280191303No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGG734170.19005590103290101No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT729180.18876413080781124No Hit
GAGGGCGCACCACCGGCCCGTCTCG722230.18696497187707495No Hit
TCGGCGACGACCCATTCGAACGTCTG707410.18312849197009484No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCG684000.17706830339908236No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCG679210.1758283075317116No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCG678480.17563933112603713No Hit
CGGGGCTACGCCTGTCTGAGCGTCGC668730.17311533118723443No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT663090.17165529429955778No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT650800.1684737600177234No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGG649330.16809321848848852No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGG647790.167694555934052No Hit
CTCGCCCGTCACGCACCGCACGTTCG625360.16188806171586279No Hit
TCGAGGGCGCACCACCGGCCCGTCTCG622490.16114509968259472No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCC608400.15749759618128906No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCG604260.15642586697650512No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCG591990.15324951013044263No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCG586890.15192926400860737No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC575350.14894188356821936No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCC570780.14775883949433952No Hit
TAAAAAGCTCGTAGTTGGATCTTGGG560200.14501997597100283No Hit
AGTGCGGTAACGCGACCGATCCCGG551360.1427315493598217No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGC540490.13991761301779246No Hit
CGGTCGGGCTGGGGCGCGAAGCGGG536710.13893907765690278No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCC508330.13159229629657243No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC507570.1313955537372401No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTC496280.12847289124400088No Hit
CCTGGCGGAGCGCTGAGAAGACGGTCG490490.12697402358803497No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC490450.12696366871649115No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT489360.12668149846692245No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCG486920.12604985130275026No Hit
CCCCTGGCGGAGCGCTGAGAAGACGGTCG484930.12553469644344592No Hit
TGGGCGAGCGCCGTTCCGAAGGGACGGGCG479560.12414455493868998No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGC470200.12172151499743938No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC469890.12164126474297488No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCC468410.12125813449585406No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT459570.11896970788467293No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG448180.11602115821257417No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGG442880.11464913773301989No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGG434390.11245131624784704No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTTG427410.11064439116345291No Hit
AAGCGAATGATTAGAGGTCTTGGGG422670.1094173388855119No Hit
CCTCGCCCGTCACGCACCGCACGTTCG419050.10848022301079747No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTG408110.10564816564356654No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGC407820.10557309282487393No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCG407350.1054514230842342No Hit
GCGGGGCTACGCCTGTCTGAGCGTCGCT389570.10084868268301246No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC388640.10060793191961896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)