FastQCFastQC Report
Sun 2 Jul 2023
SRR3356599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG17427133.485426No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT2344750.46895000000000003No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT1827860.365572No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT1466270.293254No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC1264410.252882No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT1201030.240206No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC1034960.20699199999999998No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA1009110.20182199999999997No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA887510.177502No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC879460.175892No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG830650.16613No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT714730.142946No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA709120.141824No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT654630.13092600000000001No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG574340.114868No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC566560.11331200000000001No Hit
GGAAACATCTGGAGAGAGGTTACCCGCACTCAAGTTCTCGCCAAGGTTTC555170.11103400000000001No Hit
CAGCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGGTTTCTGTGGT533070.10661399999999999No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA518660.103732No Hit
GGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGCTTGAA502490.10049799999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)