FastQCFastQC Report
Sun 2 Jul 2023
SRR3356604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4574609
Total Bases233.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG2046624.473868695663389No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT267050.5837657382303056No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT229240.5011138656877561No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT167390.36591105381902583No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC135770.29679039235921584No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC126400.2763077675053759No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA122800.26843824248148856No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT112150.24515756428582203No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA94360.206268994792779No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA90430.19767809664170208No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG86180.18838768515516843No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT85170.1861798461901334No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT82810.18102093534114064No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC80020.17492205344762798No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT79970.17481275448896288No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC67620.14781591169868288No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA61390.13419726144901128No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC60390.13201128227570924No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG56880.12433849537741914No Hit
TAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTA56250.12296132849823886No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT55320.12092836786706798No Hit
TACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGG54160.11839263202603764No Hit
TACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCT53840.11769311869058098No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA53690.1173652218145857No Hit
ACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTA49520.10824968866191625No Hit
GGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAA48520.10606370948861422No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA46090.1007517800974903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)