FastQCFastQC Report
Sun 2 Jul 2023
SRR3356619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28106094
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG12992814.622773267605239No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT1147410.40824242600199084No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT997480.3548981228056805No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT744730.26497100593202316No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT718650.25569187949061867No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC654410.23283562632360086No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT653290.23243713623102522No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG569170.20250768392078958No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC563530.20050100166889073No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC487240.17335742205942953No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA479400.17056799141139997No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT478540.17026200794745794No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA476640.16958599796898138No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA431250.15343647537790203No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA383680.13651132028520221No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT377580.13434097245956694No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT372980.13270431672220265No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC364200.12958043903218996No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA342190.12174939712362735No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG331010.1177716120923811No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG328960.11704223290507747No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT292630.10411621052715471No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT291520.1037212783818342No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG290340.10330144060572771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)