FastQCFastQC Report
Sun 2 Jul 2023
SRR3356632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27110797
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG12131634.474833403090289No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT1328040.489856495181606No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG970400.3579385733292902No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT933610.3443683341363959No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT920130.33939614538074997No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT781790.2883685049908345No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG746590.27538474800279755No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC732730.27027239368875805No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT693210.25569517561582567No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC657980.24270035292581033No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA588630.21712013851898196No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA547960.2021187352035427No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA530920.1958334164797885No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT474590.17505571673160328No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC465960.17187248312913855No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA451110.1663949606498105No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC440980.16265844194842372No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT436430.1609801438150269No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA412440.15213127079960062No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG409390.15100625776512583No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT407610.1503496927810717No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG404230.14910295702483406No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT392200.1446656105314794No Hit
CACTTGTGAGTCTTCAACTAGTTCAACAATGTCCGGACGTGGAAAGGGAG344540.1270858986550635No Hit
GCACGCCATCCCGTCCGATCTGGCAAGTTAAGCAACGTTGAGTCCAGTTA341650.12601990269780708No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG339750.12531907490583916No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT294070.10846969936000037No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC279160.10297004547671543No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA278950.10289258556286635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)