FastQCFastQC Report
Sun 2 Jul 2023
SRR3356633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25716668
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG14348115.579303664067211No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG1187200.461646119940577No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT919400.35751132300654187No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT908440.3532494956189503No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT857750.33353854395133925No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG634050.24655215831226657No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC549490.21367076014668773No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT470200.18283861657350012No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC459180.17855345801407865No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA451300.17548929744708763No Hit
CACTTGTGAGTCTTCAACTAGTTCAACAATGTCCGGACGTGGAAAGGGAG409490.15923135921029893No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT408230.15874140460187144No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA398380.15491120389313265No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA391450.1522164535467814No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA362800.14107581899801327No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT344250.13386259837394177No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA338140.1314867073759322No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG308760.12006221023656719No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG298930.11623978658510503No Hit
ACAAGTGCGGAGGAATTGACAAGAGAACCATCGAGAAGTTCGAGAAGGAG270540.10520025378093305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)