FastQCFastQC Report
Sun 2 Jul 2023
SRR3356634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27974461
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG10727043.8345832650716662No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT949300.3393452334970815No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT789730.282303919993311No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT631420.22571301731246937No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG586730.20973773185477998No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC534230.19097061423274608No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC521090.1862734727936313No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA480480.17175666047685423No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT458100.1637565063362615No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA438600.15678586264807748No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA414980.14834244706269764No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT402420.14385263758969297No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT392700.14037803981281355No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC367270.13128760550560742No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA366710.13108742291763906No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC361500.12922500991171912No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG322400.11524797564464245No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC312660.1117662284896213No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT312550.11172690690984181No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT306110.10942480714820566No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG302840.10825588382203324No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA302810.10824515975482066No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG296680.10605387535438127No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGTTATT295410.10559988984238158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)