FastQCFastQC Report
Sun 2 Jul 2023
SRR3356640.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356640.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28154375
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG12660304.496743401336381No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT2619120.9302710502364198No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG1168370.41498701356361134No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC926950.3292383510555642No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT842410.2992110462405932No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT790250.28068461828756636No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT779750.2769551801451817No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT673840.23933758074899547No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT627820.22299198614780116No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC618150.21955735121095746No Hit
TAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCT512330.18197171842742024No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA472340.16776788687370967No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA465420.16531000954558572No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC425620.15117366306302307No Hit
ACTAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAG420320.1492911847625813No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA418660.14870157835149955No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG406850.14450684840277933No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA399920.14204541922880545No Hit
CTAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGC346560.12309276977379181No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGTTATT342730.12173241281328391No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT325300.11554154549692543No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG319910.11362710058383466No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA317740.11285635003440851No Hit
AGTACTTATAGTAAGGTAAGTTTTACATCGATGTTGTATTCAGATAATCT315500.11206073656403312No Hit
ATGATGAGACTTTCACGACGGTCTTCCGATGTAATACATACCTGTGGATA314800.11181210735454082No Hit
TGACGGTAGATAAGTGTGGATGGAGTGAGAGGAGGAGTCCTGTGTATGTC310660.11034164317268631No Hit
TACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCGAACGCATAGCAC300730.10681466024374542No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT296840.10543299220813816No Hit
TAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTA292930.10404422048083113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)