FastQCFastQC Report
Sun 2 Jul 2023
SRR3356643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25331006
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG14723695.812516881485086No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG1186380.4683509213964893No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT1065220.4205202114752174No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT808120.31902404507740434No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT714810.2821877662497889No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG650880.25694992137303985No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC627620.24776749885101287No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT607370.23977334338794123No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA474300.1872408857350553No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC460600.1818324941378167No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA443170.1749515988429358No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT411250.16235044119447922No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT408160.1611305922867809No Hit
CACTTGTGAGTCTTCAACTAGTTCAACAATGTCCGGACGTGGAAAGGGAG399970.15789740052171633No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA399280.15762500707630797No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA395870.15627883077363766No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT384540.15180605144541043No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG368280.14538704068839586No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA360750.1424143991754611No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT352920.13932332572974007No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG344810.13612171581341856No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA334170.13192132993059968No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGTTATT295790.1167699379961459No Hit
AAATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCT270180.10665979866729336No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC269520.10639924841516363No Hit
AGTACTTATAGTAAGGTAAGTTTTACATCGATGTTGTATTCAGATAATCT259000.10224623530545926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)