FastQCFastQC Report
Sun 2 Jul 2023
SRR3356644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28134288
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG10824123.84730546584296No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG924960.3287660949514699No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT779460.2770498403940416No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT740620.2632446216517013No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT645340.22937847227553795No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG637530.22660250012369246No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC570440.20275615291917107No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT554830.1972077629972367No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC444680.15805624794912174No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA440570.15659539704719025No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA426790.15169745898669978No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG393160.13974407313950862No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA379250.13479992811618335No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA360840.12825631130242215No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT358660.12748145607949984No Hit
CACTTGTGAGTCTTCAACTAGTTCAACAATGTCCGGACGTGGAAAGGGAG352810.12540214275193315No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA335850.11937391129286798No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT332760.11827560733010196No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT315770.11223671272576723No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT299440.10643240731736307No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG295920.10518126493906652No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC295320.10496800203367507No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGTTATT292170.10384837178036993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)