FastQCFastQC Report
Sun 2 Jul 2023
SRR3356647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52277810
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG21629954.137501169234136No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT3118040.5964366143111198No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG1874140.35849627212769625No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT1769000.33838448856216435No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT1420030.27163150101352757No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC1316660.25185829322230596No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT1296970.24809187684028847No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC1078710.20634184943860504No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA887880.1698387901099912No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT885920.16946387004352326No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA868790.16618714517689245No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG865740.1656037236448887No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA809150.15477886315436704No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC782320.14964666653021616No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA776950.14861946206239318No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT719310.13759375153626366No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC688260.13165432905471747No Hit
TAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCT602600.11526879186408152No Hit
ACTAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAG570970.10921842364858053No Hit
TAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTA561000.10731130473904703No Hit
TGACGGTAGATAAGTGTGGATGGAGTGAGAGGAGGAGTCCTGTGTATGTC557830.10670492891725954No Hit
ATGATGAGACTTTCACGACGGTCTTCCGATGTAATACATACCTGTGGATA550750.10535062582001808No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA540850.10345689691285845No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT535580.10244882101985527No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA530330.10144457084181606No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT524760.1003791092243535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)