FastQCFastQC Report
Sun 2 Jul 2023
SRR3356648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28453981
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG13212644.6435119219345795No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC1174650.4128244831540444No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG1090880.38338396303842337No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT853560.2999791136431841No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT803360.2823365911434326No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG773830.2719584299996545No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT745230.26190711240019454No Hit
CTCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTT673380.23665581276658618No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG647290.22748662129211372No Hit
GGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGCTTGAA559990.19680550148676912No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT555160.19510802372434283No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC532470.1871337441323237No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA503100.17681181413595518No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC480570.16889376569134562No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA472060.1659029715384993No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA436640.15345480128070654No Hit
AGGCCATAAACCAGAGAGGGTGCGAGCCCCGTTCTGGATAGCGGCACTGT401280.1410277177031924No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA399920.14054975295021108No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT393220.13819507365243547No Hit
AGTACCGTGAGGGAAAGTTGCAAAGGACTTTGAAGAGAGAGTTCAAGAGA354610.1246257948931645No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT347590.1221586533005698No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT336150.1181381262607858No Hit
GAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAGCGGAGG325790.11449715946601638No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA324760.1141351714545673No Hit
ACTGCGAGTCAAAGGGTGTTAAAACCTTGCGGCGAAATGAAAGTAAAGGT320090.11249392483955056No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG298340.10485000323856265No Hit
CAGCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGGTTTCTGTGGT293120.10301546205432553No Hit
AAGGCTAAATATCGACTCGATTGCGATAGCGAACAAGTACCGTGAGGGAA288570.101416388799866No Hit
AAAGTGGGTGATAAACTTCATCTAAGGCTAAATATCGACTCGATTGCGAT287830.10115631974309677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)