FastQCFastQC Report
Tue 4 Jul 2023
SRR3356649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27462889
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG12774434.6515244627031045No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT880900.32076013561428296No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT800450.29146605806839915No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG797270.2903081318210914No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC746160.27169756248150007No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT740660.26969485985250863No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG707400.25758397086337126No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG669460.24376896400083764No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC564370.20550277867707215No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT541270.19709142763530813No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA526170.19159309859934984No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA494640.18011215061896801No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC473920.17256742362393118No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA457650.1666430651196238No Hit
CTCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTT427330.1556027117176201No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA416290.1515827413496082No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT392150.14279269744708942No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA348430.12687303218536114No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT339330.12355946965375711No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG317190.11549768125268976No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT312970.11396106214462724No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT308590.11236618259644861No Hit
GGATAGCGGCACTGTTGGTTCGCTTGCTCCTTGGAGTCGGGTTGCTTGAA304880.11101526864125622No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC299790.10916185839006232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)