FastQCFastQC Report
Sun 2 Jul 2023
SRR3356653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26939882
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG12532424.651995134945283No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT988100.3667796317741852No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT976300.36239950865412107No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT812410.301564052878925No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC727760.27014223744558347No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG722470.26817860597904625No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT672020.2494517236563991No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG667810.2478889848144101No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC559410.207651243609753No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA520110.1930632064386919No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA508220.1886496755999154No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA447350.16605492184412687No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA417210.1548670480442342No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT407950.1514297649856076No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT402260.14931765476923767No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA389790.14468882974320377No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT384600.14276231796412472No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC381870.14174895049651665No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT368290.13670809693969707No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG362110.13441410025478212No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT360470.1338053373804681No Hit
TGACGGTAGATAAGTGTGGATGGAGTGAGAGGAGGAGTCCTGTGTATGTC282590.10489652478804473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)