FastQCFastQC Report
Sun 2 Jul 2023
SRR3356654.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3356654.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27590422
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG11049704.004904310633596No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT1688330.6119261242180348No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT1031870.37399572938753894No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT847750.30726242606945264No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG747930.27108320416411175No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG721530.26151466621278935No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT672070.2435881553388346No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC631810.228996134963068No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC628760.22789067887399472No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC614660.2227802097409021No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT603730.21881869005120688No Hit
CAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAG523170.1896201515148989No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA478710.1735058637377855No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT451800.16375247903058532No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA445780.16157056242198833No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA444240.16101239770816117No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT431530.15640572659599045No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT422590.1531654716988381No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT397430.14404636507553237No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA389730.14125554150639666No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC382330.13857345132307147No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG365640.13252425062581502No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT332580.12054183150949993No Hit
ACTAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAG328220.11896157296905426No Hit
TAGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCT321790.11663105406651626No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA313520.11363363706434065No Hit
TGACGGTAGATAAGTGTGGATGGAGTGAGAGGAGGAGTCCTGTGTATGTC312670.11332555913787763No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG306940.11124875146889743No Hit
AGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTC291350.10559823985294607No Hit
CACTTGTGAGTCTTCAACTAGTTCAACAATGTCCGGACGTGGAAAGGGAG278900.10108580434181108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)