FastQCFastQC Report
Sun 2 Jul 2023
SRR3488641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3488641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51763739
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC15891723.070048707262047No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA13420662.592675927061606No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA12607722.435627766379086No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA9311101.7987688254127083No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC8630961.6673756893797798No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC7628561.473726617777746No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG6357711.2282169184107816TruSeq Adapter, Index 10 (100% over 49bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG4789460.9252538731794472No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC4738290.9153685749014382No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC4675630.9032635760720452No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC4181660.8078357709051891No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC3772460.7287842943493706Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT3415950.6599117579199602No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC3333600.6440029380412415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA3254180.6286601514623973No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA3170270.6124499623182166No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA2947750.5694623411960252No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2897490.5597528416562026No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2444410.4722243885821308No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC2411000.4657700634801516No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG2345910.4531956240641736No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2277910.44005901505685285No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2184560.4220251554857736No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2182750.42167548986366693No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1942560.37527428225383797No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG1923360.37156512206353565No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAGATCGGAAGAGC1885300.3642124847279676No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1798270.34739955705286285No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAGATCGGAAGAGCACAC1778040.34349141587318494No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC1776180.3431320909797494No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA1738250.33580456774963646No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT1734800.33513807802794154No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1679180.3243931046016595No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1656600.3200309776695227Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC1609120.3108585336155875No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGTC1561860.3017285903554996No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCAGATCGGAAGAG1508320.29138544261650035No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1428920.2760465197461876No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC1394610.26941832776028796No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCT1391750.26886581744027416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG1384880.2675386335596816No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG1383710.26731260661058504No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGTCT1378010.26621144967908905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1348400.2604912291981072Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT1325150.255999668030163Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT1301440.25141924156599277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGT1288780.24897351406551216No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA1286590.2485504379813058No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTG1271900.24571254406487134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC1267960.24495139348415304No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1264530.24428876747098968No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1255110.24246896075262261No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT1226300.23690328861290336No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1217070.2351201871255861No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT1206340.23304730749840155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1196080.23106522502170873No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1156920.22350008371690458No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT1136230.21950307724100068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC1135300.2193234147942829No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1133000.21887908831315298No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC1102250.21293863644587188No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1101850.21286136227524058Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA1096910.21190702626794405No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCT1093800.21130621959128568Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1028130.19861973262789226No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1025730.19815608760410447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1000070.19319894955810668No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC938770.1813566829088602No Hit
AGGATAGCTGGCGCTCTCGCTCCCGACGTACGCAGATCGGAAGAGCACAC926500.1789862977247451No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG904140.17466667158645555No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC898110.17350176346418872No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCAC865610.1672232371003957No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT865560.1672135778290668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCAGATCGGAAGAGCACA857170.16559275210007532No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA854150.16500933211180902No Hit
TCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAA830270.1603960641251205Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT829640.16027435730637618No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACA826390.15964650466999689No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG810140.15650724148810038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG795110.15360366452662935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGA760400.14689819837009843Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA758820.1465929653961048No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC751360.14515180211383108No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC727120.14046898737357438No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC667150.12888365734167695No Hit
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC656420.12681077771449237No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG643620.12433800425429083No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG634390.12255490276697362No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG624470.1206385033353174No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTG622410.12024054135656623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC608280.1175108312790156Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT587990.113591098973743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG586980.11339598169289897No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA573430.11077831916276373No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG553480.10692426990252771No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG547440.10575742992599511No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT546870.10564731423284551No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGT539920.10430467551812668No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC518050.10007971023886045No Hit
TTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTG518010.10007198282179731Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)