FastQCFastQC Report
Sun 2 Jul 2023
SRR3488644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3488644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59561713
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC58275159.783994963341636Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG20297173.4077545754938243TruSeq Adapter, Index 8 (100% over 49bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA9664431.622591009093375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC7135661.1980280016459568No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT5586730.9379733588253246No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA5266910.8842777910030896No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC5141700.8632558972909324Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTG4546740.763366224876709Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGTCT3665000.6153281723109608Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT3173140.5327482774043117No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG3168580.5319826849170708No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2878840.48333734122119687No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC2848390.4782249966517921No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2586620.43427562266384107No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2541980.42678087515716684No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA2273350.38167975457656833No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC2034920.34164900529304787No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2031640.34109831596012025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1990110.3341257159611914No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC1914590.3214464298567101No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA1857350.31183622942476485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1753530.29440556889288927No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT1652050.27736777818999264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1602950.26912422750500814No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT1407160.23625243954954753Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT1364440.22908004677434313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG1324450.22236600213294738No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1314050.2206199140041523No Hit
ACGGCGGTAACACGGGTTCGAATCCCGTACGGACTACAGATCGGAAGAGC1309980.2199365891306719No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA1253420.21044055599945558No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT1252140.2102256528451423No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT1251840.21017528491835014No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT1227540.2060954828481847No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC1222890.20531477998290615No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1185770.19908258850782212No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG1176910.19759505573656017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA1153950.1937402304060664No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1111360.1865896637324719Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC1105900.18567296746485448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCT1102130.18504001051816626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG1100220.18471933471758947No Hit
AGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1096960.18417200324644792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACT1081000.1814924295411047Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1027570.17252190177941992No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCAGATCGGAAGAGCACACGTCTG925400.1553682648449013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATCATGGGGGTTCGAGTCCCCCCCCGCGCACCAGATCGGAAGAGCAC916490.15387233741917394No Hit
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGTC907450.1523545838918367No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT903970.15177031594104756Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG890400.14949200671914858No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT809540.1359161715177668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA801990.13464857869349728No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGT794920.13346157455209523No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC756680.12704134281698715No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA747330.12547154243196465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA744760.12504005719244507No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC729870.12254012909266059Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCAAGATCGGAAGA723770.12151598124788654No Hit
ACGATCATGGGGGTTCGAGTCCCCCCCCGCGCACAGATCGGAAGAGCACA717880.120527090951867No Hit
CGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAAC701940.11785087510830994Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGA692120.11620216497131304Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC687630.11544832500032362No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG684670.11495136145597425No Hit
ATGGAAGAGGTCGGGGGTTCGAATCCCCCTAGCTCCACCAGATCGGAAGA673400.11305920633948188No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT642990.10795357749364932Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG633240.10631661987290392No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT632410.10617726860877894No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGT610810.102550777879743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)