FastQCFastQC Report
Sun 2 Jul 2023
SRR3488645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3488645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36632448
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT11616093.1709838228665475No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8548162.3334940651522933No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA7610652.07757068269093Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG6443461.7589487876977263No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4374581.1941817265392694TruSeq Adapter, Index 9 (100% over 49bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG3995191.090615074373408No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT3963631.0819997615228991No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC3528010.9630833298391634No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT3516870.9600423100307138No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3500230.9554998890601032No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3311480.9039745309950349No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG2640890.7209155118434891No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA2332180.6366432295215433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2210620.6034595340174917No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG1821440.49722038778298405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1654440.45163238885918844No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1587180.43327161755610766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1515560.4137206446044774No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC1423830.3886800030399279No Hit
GCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGA1299350.35469920000978367Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC1238300.3380336471097973No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1191320.3252089513646481No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1177410.3214117713345283No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1148320.3134707240968444Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1097310.29954591077287546No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTG1055760.2882035074478233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1028640.28080023480822247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCT989270.27005293230744504Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT923700.25215350063419184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC915310.24986318140682273Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC896940.2448485015252052No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG869610.23738790265941276No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC864630.23602845215258342No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC811840.22161773081613328Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC797120.21759943534213166No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA785420.21440554559717112No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG775540.21170848314587112Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT748020.20419601769447676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA745500.20350810298017757No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC725250.1979802168831305No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT709820.19376810416819537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT674440.18410999996505828No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA628610.17159923355381546Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG622440.16991493443190037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG598100.16327055183426453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT597750.1631750081239452Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC585670.15987738520778083No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA571370.1559737421861624Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGACCGGCGCAAGACGGACCAGAGCGAAAGCAGATCGGAAGAGCACACGT557980.15231851281137423No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA529560.14456036353344445Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT502670.1372198767606249Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC498590.13610611008033097No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA495800.13534449021807116Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA494620.13502237142328025No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC487690.13313060595895748No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT484630.13229528094873702No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT457330.12484287154382913Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTGA450620.12301116212599277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC450310.1229265376968528Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT424350.11583992421145319No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT421480.11505646578683466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA414980.11328208259518992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT412350.11256413985764752No Hit
GGACCGGCGCAAGACGGACCAGAGCGAAAGAGATCGGAAGAGCACACGTC410880.11216285627430632No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC407070.11112279474197301No Hit
TAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTGAA400070.10921192053558637Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC394090.10757948799927321No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC387480.10577507678438526No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA383310.10463674172143778Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA373320.10190965124689455Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT371160.10132001006320955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC370900.10124903473554374No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC368440.10057749894301357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)