FastQCFastQC Report
Mon 10 Jul 2023
SRR3494962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66801609
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG9753951.460136985622607TruSeq Adapter, Index 6 (100% over 49bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG8046301.2045069153948074Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5559550.8322479178607808Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC3578810.5357370957936058No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3039830.4550534104650084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2958500.4428785540180626Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC2586550.3871987574431029No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2461310.3684507060301496No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2155800.3227167776752204Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2016080.30180111380251334Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1872330.28028217104770636No Hit
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1693650.2535343123247226TruSeq Adapter, Index 6 (100% over 37bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA1658110.24821408119076893Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1500090.22455896234475428No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1462570.2189423311645083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1457210.21813995528161606No Hit
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1453940.21765044611425452Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1433510.21459213654569306Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1422580.2129559484113624TruSeq Adapter, Index 6 (100% over 35bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1343610.20113437686807814Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1180080.1766544275902097No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1179080.1765047305971328Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1150710.1722578269035406Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1105430.16547954705701773Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1073430.16068924327855635No Hit
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1062380.1590350915050564Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA1046800.15670281235291803TruSeq Adapter, Index 6 (100% over 38bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC975810.14607582281438758Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA933840.13979304001494933Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG876940.13127528110887268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT863360.12924239594288814No Hit
GACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG850190.12727088654406513Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT792850.11868726096103464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG785600.11760195776122698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC735360.11008118082904261TruSeq Adapter, Index 6 (100% over 35bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT715780.10715011370459654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT714070.10689413184643501No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC687550.10292416759003514No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTG676210.10122660368854289Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG670430.10036135506855831Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)