FastQCFastQC Report
Sun 2 Jul 2023
SRR3494963.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494963.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25717173
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG3880221.5088050307862377TruSeq Adapter, Index 6 (100% over 49bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG3214481.249935208663876Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2185590.8498562419749636Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1449180.5635067275862709No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1203740.4680685548135481Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1172100.4557654917980293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1026520.39915740349843276No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1011380.3932702867457477No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT831110.32317315748507813Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA806830.3137319953480112Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG761870.2962495138948593No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA659620.2564900893266923Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA643020.25003525854105346TruSeq Adapter, Index 6 (100% over 37bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC590140.22947312288174132Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT588710.22891707420562907No Hit
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC574740.2234849063697631Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT572680.22268388519997903No Hit
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC568320.2209885200056787TruSeq Adapter, Index 6 (100% over 35bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG564000.2193087086205004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC529140.2057535639706588Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC460770.17916821572884392Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC459160.17854217491168256No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC455870.1772628741113963Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC439270.17080804332575747No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG429640.16706346377963083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG422590.1643221049218746Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA406060.15789449330219926TruSeq Adapter, Index 6 (100% over 38bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC387540.1506930796787034Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA364170.14160576669916244Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG346150.13459877568969186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT340260.13230847729647424No Hit
GACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG332620.12933769975416815Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG317660.1235205751425322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT302540.11764123529440815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC290560.11298286946236276TruSeq Adapter, Index 6 (100% over 35bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT287490.11178911461224764No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT279820.10880667171310003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC278770.10839838422364698No Hit
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG278730.10838283041452496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA262470.10206020700642329Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTG258400.10047760692825762Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)