FastQCFastQC Report
Tue 4 Jul 2023
SRR3494964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76173806
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG37918474.977888330799698TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC5501660.7222509007886517No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG4049720.5316420712915408No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3925990.515398954858577No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3309130.43441836160845104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2561870.3363190228410013Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2485690.32631820970058917Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2455700.32238116078905127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC2373560.31159792645781675No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2269660.2979580671077404Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA2196760.2883878481797273Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG2067850.271464707960109TruSeq Adapter, Index 12 (100% over 37bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2057440.27009809645063554Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1894950.2487666167028598Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1849380.2427842452824269No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG1743720.22891333537935601Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1686930.2214580166835828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1468960.19284319336754685Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1449630.1903055756463055Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1406140.18459626397032072Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1321950.17354390825633684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1231210.161631676904788Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1215870.15961786128948316No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1194790.1568505058024802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1173010.15399125520917256TruSeq Adapter, Index 12 (100% over 35bp)
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT1140000.14965774455329173TruSeq Adapter, Index 12 (100% over 38bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1100180.1444302257917899No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC1089810.1430688654312481No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1071080.14061001494398218No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG1032910.13559910607591275TruSeq Adapter, Index 12 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1025870.13467490386393455No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC984340.12922289848560278Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG972830.1277118803805077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT953360.12515588363800542TruSeq Adapter, Index 12 (100% over 36bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT920050.12078298936513687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG861440.11308874339297159TruSeq Adapter, Index 12 (100% over 37bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA793510.1041709797197215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT787530.10338593295443317No Hit
ACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC785150.10307348959299736Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC784590.10299997350795365No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC772860.10146007408373425TruSeq Adapter, Index 12 (100% over 50bp)
AAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGA766440.1006172646801973Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)