FastQCFastQC Report
Thu 6 Jul 2023
SRR3494965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35271172
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG16417284.65458873892821TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2640110.7485177980476521No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1968440.5580874942289981No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1947670.5521988325196565No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1561950.4428404023546481Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1189030.33711099818287865Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1185280.33604780697392195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1134270.3215855713555535Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1129750.320304071551691Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1122970.3183818218458973No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA1044350.29609166375305024Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC989800.28062577563342667Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG953850.27043331591022834TruSeq Adapter, Index 12 (100% over 37bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC917820.26021817477457226No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA879760.249427492797801Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG820670.23267443452120048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG807810.22902839746861825Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC717420.20340123656792578Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA702040.19904073502292466Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA656300.18607263745021005Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC615930.17462702968872143Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG610870.17319243035076917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC587510.1665694579131082No Hit
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT562460.15946734063727738TruSeq Adapter, Index 12 (100% over 35bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG560590.15893716262107763Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC532680.1510241848498825No Hit
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT532370.15093629437660874TruSeq Adapter, Index 12 (100% over 38bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT516750.1465077485942344No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC502000.142325863172338No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG488380.13846435270140728TruSeq Adapter, Index 12 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT465470.13196896320882107No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC456020.12928972136225017Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG449730.12750639530776012Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT443250.12566920089868294TruSeq Adapter, Index 12 (100% over 36bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT436890.12386602860829235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG414250.11744718888275106TruSeq Adapter, Index 12 (100% over 37bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA378350.10726890504233882Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC374780.10625674701141204TruSeq Adapter, Index 12 (100% over 50bp)
ACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC373430.10587399817618762Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT366500.10390922082203563No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG363370.10302181055962642No Hit
AAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACACGTCTGAACTCCAG362530.10278365572882013Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC362530.10278365572882013No Hit
AAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGA361260.10242358830605344Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)