FastQCFastQC Report
Sun 2 Jul 2023
SRR3494966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52416474
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG46174308.809119819849004TruSeq Adapter, Index 11 (100% over 49bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3844490.73345070864553Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC2903110.5538545000184484No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2888180.5510061588652453No Hit
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT2683020.5118657924224357TruSeq Adapter, Index 11 (100% over 37bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2678560.5110149148910703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1986630.37900870630863115Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG1730060.3300603546892528TruSeq Adapter, Index 11 (100% over 50bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1635360.31199351562640404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1517130.28943762985659816No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1501360.2864290337423307Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1472760.28097273387752103No Hit
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA1408550.26872276834187664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1373490.262034031514596No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1342500.2561217681296151Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA1269260.2421490617625291TruSeq Adapter, Index 11 (100% over 38bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1243640.2372612854500667No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1228360.23434617139642014No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1192240.22745520807065353TruSeq Adapter, Index 11 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1146420.21871368150402484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC917450.17503085003390345TruSeq Adapter, Index 11 (100% over 50bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA917320.1750060486708816Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG912290.17404642670165107TruSeq Adapter, Index 11 (100% over 35bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG834890.15928007671786545Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA774540.14776652088425482Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG771120.1471140542570643No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG731340.13952483717237446Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC674240.1286313154143104TruSeq Adapter, Index 11 (100% over 36bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC657760.12548726570200047Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA650250.12405451003819905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT642890.12265037133173055No Hit
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC597960.11407863871194389Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT585560.11171297023908934No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG527880.10070879624600464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC527530.10064202334556116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)