FastQCFastQC Report
Sun 2 Jul 2023
SRR3494967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25897822
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG21489478.297790447397468TruSeq Adapter, Index 11 (100% over 49bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1901860.7343706354920503Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1557330.6013362822557047No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1481490.5720519663777132No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1329170.5132362095932237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT1326750.5123017680791845TruSeq Adapter, Index 11 (100% over 37bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1009030.3896196367401089Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG896080.3460059305373247TruSeq Adapter, Index 11 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC812440.3137097783744131No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG810550.3129799872746056Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT758550.2929010787084721No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC743340.28702799795287803Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA717380.27700398898409295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG707120.273042265870852No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG694830.2682966930578178Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA639300.24685473550632944TruSeq Adapter, Index 11 (100% over 38bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC636310.2457001982637768No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC625220.24141798487919175TruSeq Adapter, Index 11 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT605650.23386136486689885No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC562040.21702211097133958No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC480640.18559089640819987TruSeq Adapter, Index 11 (100% over 50bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG471140.1819226342663101TruSeq Adapter, Index 11 (100% over 35bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA467180.18039354815242764Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG421260.16266232735710362Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA410050.1583337780296737Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG398250.15377741031658956No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG369440.14265292270523752Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC340570.13150526712246305TruSeq Adapter, Index 11 (100% over 36bp)
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC335030.12936609109445574Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT328070.1266786064094502No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA325740.12577891685254458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC312600.12070513111102547Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT298360.11520659922676123No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC276580.10679662560040763No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT264980.10231748445873169Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG262370.10130967770185463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)