FastQCFastQC Report
Sun 2 Jul 2023
SRR3494968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3494968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62537607
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1453504123.242080561221346TruSeq Adapter, Index 5 (100% over 49bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG14218712.2736255322337486TruSeq Adapter, Index 5 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC3992630.6384366450094581TruSeq Adapter, Index 5 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3753580.6002116454503927No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT3599180.575522501204755TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2775210.4437665803234204TruSeq Adapter, Index 5 (100% over 50bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2519440.40286798949630426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG2506360.40077644800191986TruSeq Adapter, Index 5 (100% over 37bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2257340.3609572077166304Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT2155930.3447413649837929TruSeq Adapter, Index 5 (100% over 38bp)
AGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGA1741390.2784548503750711Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1715580.27432773371069347No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1558360.24918766079424814Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC1540230.24628860519079343TruSeq Adapter, Index 5 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1398080.2235582822988414No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1147340.18346400750511607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1044190.1669699321881632Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1006590.1609575499107281No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA939610.150247194460127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA852650.13634196140571864Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA806190.12891283160227093Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTGATCTCGTATG775110.12394302199634853TruSeq Adapter, Index 5 (97% over 49bp)
GGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA749410.11983349474820808Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC749050.1197759293859773No Hit
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC741940.11863901348191977TruSeq Adapter, Index 5 (100% over 35bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC721850.11542654646187532No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG715880.11447192087154855No Hit
GCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC686370.10975316020646585Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA685390.109596454498171Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT665590.10643035957547911No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC656950.10504879088194086No Hit
GGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG648420.10368481160463974Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)